绿脓杆菌MSHA菌苗对实验性肝纤维化的防治作用  

Protective Effect of PA-MSHA Vaccine on Experimental Hepatic Fibrosis

作　　者：贾乐文[1,2] 李春梅[1,2] 王英德[1,2] 侯金萍 牟希亚[1,2] 

机构地区：[1]大连医科大学附属一院内科 [2]大连医科大学菌毛研究室

出　　处：《基础医学与临床》1999年第1期73-77,83,共6页Basic and Clinical Medicine

摘　　要：为研究绿脓杆菌ＭＳＨＡ菌苗（简称ＰＡ－ＭＳＨＡ菌苗）对大鼠实验性免疫损伤性肝纤维化的防治作用及其机理。本文用人血清白蛋白（ＨＳＡ）致敏、攻击的方法建立一组肝纤维化大鼠模型，作为损伤组，另外建立一组经ＨＳＡ致敏同时皮下注射ＰＡ－ＭＳＨＡ菌苗的大鼠模型作为保护组，并设正常对照组，结果发现，保护组血清β－ＮＡＧ酶活性（７６．７±５．３ｕ／Ｌ）较损伤组（９４．３±６．３ｕ／Ｌ）显著降低（Ｐ＜０．０１）；用菌苗保护后的大鼠血清肿瘤坏死因子（ＴＮＦ）（０．３４８±０．０７５ｎｇ／ｍＬ）显著高于损伤组（０．２１１±０．０２９ｎｇ／ｍＬ，ｐ＜０．０１）：ＰＡ－ＭＳＨＡ菌苗能明显抑制Ⅰ、Ⅱ、Ⅲ型胶原及层粘蛋白在肝内沉积（Ｐ均＜０．０１）；肝细胞超微结构显示了保护组肝细胞萎缩也减轻。提示，ＰＡ－ＭＳＨＡ菌苗对实验性肝纤维化具有显著预防和治疗作用。To study on the mechanism of anti-hepatic fibrosis of Pseudomons Aeruginosa MSHA vaccine (PA-MSHA) preparation on the HSA-immune-induced liver fibrosis of rat models . These rats were divided into the injury group which were established by HSA infection, the protection group pro- tected by PA-MSHA vaccine and the normal group.The results showed:PA-MSHA could remarkablly decrease the activity of serum β-NAGase (76.7 ±5.3u/L) and significantly elevate serum TNF level (0.384 ± 0.075ng/mL) by stimulating the immune cells in comparison with the rats induced HSA (P<0.0 1) . Immunohistochemical finding indicated that PA-MSHA could significantly suppress the deposition of collagen type ⅠⅡD ,Ⅶ and Laminin in the liver with the rat models. PA-MSHA could also protect the hepatocytes from immune injury at the ultrastructrue level . It sugguested that PA- MSHA may have protectively effect on the experimental hepatic fibrosis .

关 键 词：绿脓杆菌 MSHA菌苗 肝纤维化 防治 

分 类 号：R573.2[医药卫生—消化系统]