对羟基苯甲酸酯的细胞毒效应  

Cytotoxicity of Parabens

作　　者：董力[1] 张宏伟[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京市100021

出　　处：《职业与健康》2010年第12期1324-1325,共2页Occupation and Health

基　　金：中国疾病预防控制中心环境与健康相关产品安全所青年基金

摘　　要：目的探讨对羟基苯甲酸酯对人角质形成细胞(HaCat)的毒作用。方法采用人角质形成细胞株,分别用对羟基苯甲酸甲酯、乙酯、丙酯、丁酯作用72h,通过MTT比色法测定其对细胞增殖活力的影响。结果对羟基苯甲酸甲酯、乙酯在200μg/ml时吸光度值明显减小(P<0.05);丙酯、丁酯从25μg/ml开始吸光度值亦明显减小(P<0.05),且随剂量的增加,吸光度值也越来越小。呈现明显的剂量—效应关系。从增殖率来看,随剂量增加,甲酯、乙酯、丙酯、丁酯的增殖率逐渐下降,从50μg/ml开始,增殖率由大到小依次为甲酯、乙酯、丁酯、丙酯。结论对羟基苯甲酸酯类在一定的剂量具有明显的细胞毒作用,毒性最大的为对羟基苯甲酸丙酯,毒性最小的为对羟基苯甲酸甲酯。[Objective]To study the cytotoxicity of parabens on HaCat.[Methods]The effects of methylparaben, ethylparaben, n-propylparaben, n-butylparaben on the proliferation of HaCat cell line at 72 hours was evaluated by MTT assay.[Results]Methylparaben, ethylparaben could significantly decrease absorption value at 200 g/ml; n-propylparaben, n-butylparaben could significantly decrease absorption value from 25 μg/ml to larger dose （P0.05）, and showed a obvious dose-effect relationship. With the dose increasing, the proliferation rate of methylparaben, ethylparaben, n-propylparaben, n-butylparaben decreased gradually. Starting with the dose of 50 μg/ml, the proliferation rate from the biggest to the smallest was methylparaben, ethylparaben, n-butylparaben n-propylparaben.[Conclusion]Parabens can induce obvious cytotoxicity on HaCat at a certain level. N-propylparaben is the most toxic to HaCat, while methylparaben is the least toxic to HaCat.

关 键 词：对羟基苯甲酸酯 防腐剂 人角质形成细胞株 

分 类 号：R114[医药卫生—卫生毒理学]