3种鲆鲽鱼精子和卵子表面形态结构的比较研究  

A Comparative Study on Surface Morphology of Sperm and Mature Egg in Three Species Flatfish

作　　者：张世奎[1] 刘海金[2] 李忠红[3] 张晓燕[3] 王玉芬[4] 

机构地区：[1]上海海洋大学,上海200090 [2]中国水产科学研究院,北京100141 [3]东北农业大学,黑龙江哈尔滨150030 [4]中国水产科学研究院北戴河中心实验站,河北秦皇岛066100

出　　处：《水产科学》2010年第2期63-68,共6页Fisheries Science

基　　金：国家科技支撑计划项目(2006BAD01A1207);农业科技跨越计划项目(2006跨18);国家鲆鲽产业技术体系项目

摘　　要：使用扫描电镜对牙鲆、大菱鲆和条斑星鲽的精子和卵子表面形态结构进行比较研究。观察结果表明,3种精子形态结构在核前凹陷位置、精子头部形状、中段线粒体数及鞭毛长度4个方面存在明显差异;3种卵子表面形态结构中,牙鲆和大菱鲆比较相似,而条斑星鲽成熟卵子壳膜具有4点特异性：外表面粗糙、有点状和斑块状突起、网纹较深;壳膜上微小孔孔径差距大;卵膜厚度最厚为（3.87±0.24）μm,约为牙鲆和大菱鲆卵膜厚度的2倍;受精孔的管道螺旋层为4-6层,约为前两者的1/2。条斑星鲽与牙鲆和大菱鲆的精卵表面形态结构差异明显。The surface morphological structures of sperm and mature egg of three species flatfish Japanese flounder Paralichthys olivaceus,turbot Scophthalmus maximus L,and Verasper moseri were studied by scanning electron microscopy.Comparing results demonstrated that there are four obvious differences in sperm surface structures among three species flatfish,including the location of nucleus anterior pit,the shape of sperm head,the number of mitochondria in midpiece and length of the flagellum.But in the surface morphology of mature egg of three species flatfish,it is similar essentially among Japanese flounder and turbot,excepting stripe stars flounder.Four structure characteristics of the shell membrane are observed in V.moseri,they are a rough outer surface,various microspores in size,diameter as well as the most thick shell membrane（3.87±0.24） μm which is twice thicker than Japanses flounder and turbot and spiral pipe layer in micropyle only for 4-6 layers about half of Japanses Flounder and turbot.It was concluded that the difference was obvious in the surface morphology of sperm and mature egg between three species of Flatfish,and stripe stars flounder as genetic distance of the farthest Pleuronectidae fish,have significant level.

关 键 词：牙鲆 大菱鲆 条斑星鲽 精子 成熟卵子 表面形态结构 扫描电镜 

分 类 号：Q959.4[生物学—动物学]