低电压电泳芯片及其在生化样品分析中的应用(英文)  

Low-Voltage-Driven Electrophoresis Microchip and Its Application in Biochemical Analysis

作　　者：徐溢[1,2,3,4] 梁静[1,2,3] 胡小国[1,2,3] 马亮波[1,2,3] 温志渝[2,3,4] 

机构地区：[1]重庆大学化学化工学院,重庆400030 [2]重庆大学微系统中心,重庆400030 [3]新型微纳器件与系统技术国家重点学科实验室,重庆400030 [4]微纳系统及新材料技术国际研发中心,重庆400030

出　　处：《纳米技术与精密工程》2010年第3期245-250,共6页Nanotechnology and Precision Engineering

基　　金：国家自然科学基金资助项目(20675089,90307015);国家高技术研究发展计划(863)资助项目(2006AA04Z345);全国博士学位论文作者专项基金资助项目(200941);国际科技合作项目(2006DFA13510);重庆大学研究生科技创新基金资助项目(200904A1B0010307)

摘　　要：为了解决微流控电泳芯片集成化问题,设计并制作出一种具有管道两侧微阵列电极结构的硅-PDMS复合低电压电泳芯片.通过电路控制程序在微侧壁阵列电极上施加交替循环的低电压,以实现芯片微管道中低电压电泳过程;并对硅-PDMS芯片的电绝缘性、伏安曲线及电渗流等性能进行了测试和评价.以pH为10.0、10mmol/L的硼砂作为缓冲体系,分离场强150V/cm、切换时间3s的条件下,完成了10-4mol/L的苯丙氨酸和精氨酸的低电压电泳分离,分离度达1.6,实现了两种氨基酸的完全分离.在此基础上,将系统用于牛血清白蛋白和α-乳白蛋白的分离,并初步实现了该两种蛋白质的芯片电泳分离.A new SOI-PDMS hybrid low-voltage-driven electrophoresis microchip with integrated array-electrodes on the microchannel sidewall was designed and constructed. A specially integrated circuit（ IC） was proposed to power a DC voltage to particular sets of these electrode pairs in a controlled sequence so that the moving electric field could be formed,and the low-voltage-driven electrophoresis could be realized in the microchannel. Using the mini laser-induced fluorescence （ LIF） as detection mode,a set of low-voltage-driven electrophoresis separating and detecting system was finally established. The electrical properties of the microchip,such as insulation,volt-ampere characteristic curve and electroosmotic flow, were tested and evaluated. Fluorescein-5（ 6）-isothiocyanate （ FITC） labeled 10-4 mol/L arginine and phenylalanine were successfully separated under the optimal conditions,including 10 mmol/L borax buffer （ pH = 10） ,150 V/cm separation electric field strength and switching time of 3 s. The separation was completed with a resolution of 1. 6. Finally,the system was applied to the protein analysis,and the separation of BSA and α-lactoglobulin from their mixture was realized preliminarily by low-voltage-driven electrophoresis on microchip.

关 键 词：微流控芯片 MEMS技术 低电压芯片电泳 电极阵列 

分 类 号：TN492[电子电信—微电子学与固体电子学]