职业性肺癌中HSPs与多种瘤抑制基因、k-ras表达的相关性  

Correlation of heat stress proteins,k ras and MTS1 expression in occupational lung cancer

作　　者：张永幸[1] 贺涵贞[1] 张国高[1] 张招弟 鲁翠荣[1] 杨木兰 陈荣安[1] 潘秋萍[1] 何骁生[1] 陈胜[1] 

机构地区：[1]同济医科大学职业医学研究所

出　　处：《中华劳动卫生职业病杂志》1999年第1期17-19,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家博士后科研基金;湖北省科委资助

摘　　要：目的观察ＨＳＰ２７、ＨＳＰ７０与多种瘤抑制基因（ＭＴＳ１）、ｋｒａｓ基因及其蛋白产物在职业性肺癌（ｏｃｃｕｐａｔｉｏｎａｌｌｕｎｇｃａｎｃｅｒ，ＯＬＣ）中的联合表达的情况。方法采用免疫组化和原位分子杂交技术。结果ＯＬＣ中的ＨＳＰｓ表达较正常肺组织高；ＯＬＣ中的ＨＳＰｓ与ｋｒａｓ都有基因扩增和蛋白产物的过表达；ＭＴＳ１的表达与ＨＳＰｓ、ｋｒａｓ的表达呈负相关。结论ＭＴＳ１表达的变化可能是ＯＬＣ癌变的重要分子学改变；ＨＳＰｓ与ｋｒａｓ在ＯＬＣ的癌变过程中有协同作用；ＯＬＣ中以上３种基因在基因水平及蛋白产物表达上的改变基本一致。Objective To study the expression of heat stress proteins HSP27,HSP70 and antioncogene MTS1,oncogene k ras,and their protein products in occupational lung cancer(OLC). Methods In situ hybridization and immunohistochemistry were conducted in the study. Results The expression of HSPs was higher in OLC than in normal lung tissue.HSPs and k ras were overexpressed and amplified in OLC.The expression of MTS1 correlated negatively with HSPs and k ras expression. Conclusion HSPs and k ras might play a synergetic role in the process of carcinogenesis;change of MTS1 expression might be an important molecular alteration of OLC;the expression of the above three genes in OLC are consistent with their protein products.

关 键 词：职业性 肺癌 热应激蛋白 癌基因 抑癌基因 

分 类 号：R734.202[医药卫生—肿瘤] R73－31[医药卫生—临床医学]