金属离子对青霉葡萄糖氧化酶的效应研究  

Effects of Metal Ions on the Activity of GOD from Penicillium amagasakiense

作　　者：张茜[1] 闫冰[2] 康劲翮[1] 王勤[1] 石艳[1] 陈清西[1] 

机构地区：[1]厦门大学生命科学学院,滨海湿地生态系统教育部重点实验室,福建厦门361005 [2]厦门大学附属第一医院,福建厦门361004

出　　处：《厦门大学学报（自然科学版）》2010年第3期396-399,共4页Journal of Xiamen University：Natural Science

基　　金：国家科技支撑计划课题(2007BAI07A24)

摘　　要：研究了几种金属离子对青霉(Penicillium amagasakiense)葡萄糖氧化酶(简称GOD,EC.1.1.3.4)活力的影响.金属离子对酶活力的影响表明:Na+、K+、Ca2+、Pb2+、Mn2+等对酶活力基本没有影响;Al3+、Zn2+对酶有一定的激活作用;Ag+、Hg2+、Mg2+、Cd2+、Cu2+、Co2+等对该酶活力有不同程度的抑制作用.其中Ag+、Cu2+、Hg2+对酶的抑制作用较强,随着抑制剂浓度增大,酶活力呈指数下降,导致酶活力下降50%的抑制浓度为0.08、10.0和31.25μmol/L.进一步研究了Ag+的抑制动力学,结果显示:Ag+对酶的抑制作用为竞争性可逆抑制,抑制常数(KI)为0.065μmol/L.该研究对青霉葡萄糖氧化酶的应用具有一定的参考价值.Effects of metal ions on the activity of GOD from Penicillium amagasakiense were studied.The results showed that Na＋,K＋,Ca2＋,Pb2＋ and Mn2＋ effected lightly on the enzyme activity,while Al3＋ and Zn2＋ enhanced the enzyme activity lightly.Ag＋,Hg2＋,Mg2＋,Cd2＋,Cu2＋ and Co2＋ inhibited the enzyme activity.Ag＋,Cu2＋ and Hg2＋ inhibited it obviously.They were potential potent inhibitor,and the inhibitor′s concentration leading to 50% of enzyme activity lost（IC50） for Ag＋,Cu2＋ and Hg2＋ were estimated to be 0.08,10.0 and 31.25 μmol/L,respectively.The inhibitory kinetics and mechanism of Ag＋ on the enzyme were studied.It showed that the inhibition of Ag＋ to GOD was reversible and competitive.The inhibition constant（KI） was determined to be 0.065 μmol/L.The data from this study can be useful to the application of GOD.

关 键 词：葡萄糖氧化酶 青霉 金属离子 抑制机理 

分 类 号：TQ925.4[轻工技术与工程—发酵工程]