皮质发育不良大鼠脑皮质中尿激酶型纤溶酶原激活剂受体的表达  

Expression of uPAR in the rat cerebral cortex with cortical dysplasia

作　　者：孟强[1] 梁秦川[1] 井晓荣[1] 刘备[1] 王超[1] 郭恒[1] 游宇[1] 张华[1] 高国栋[1] 

机构地区：[1]第四军医大学唐都医院神经外科,西安710038

出　　处：《立体定向和功能性神经外科杂志》2010年第2期85-89,共5页Chinese Journal of Stereotactic and Functional Neurosurgery

基　　金：国家自然科学基金资助项目(编号:30772221)

摘　　要：目的观察γ-射线照射诱导的皮质发育不良(CD)模型大鼠皮质尿激酶型纤溶酶原激活剂受体(uPAR)的表达,探讨其在癫痫发病机制中的作用。方法建立CD模型,按大鼠的不同年龄分为新生组、幼年组(又分为2周龄和4周龄)和成年组(2.5个月龄)。分别利用苏木精-伊红染色、免疫组化及免疫荧光染色,光镜下观察不同组之间大脑皮质的病理变化及uPAR阳性细胞表达的情况。结果免疫组化显示各组中均有uPAR阳性表达细胞;随着年龄的增长,阳性表达细胞逐渐减少,但CD模型组阳性表达细胞在皮层中所占比例明显高于正常对照组;免疫荧光双标显示uPAR阳性表达细胞主要是神经元。结论uPAR在CD发病过程中对神经组织的迁移发挥着关键作用,而较高比例的阳性神经元表达可能在CD致癫过程中发挥着重要作用。Objective To explore the epileptogenesis effect of the uPAR in the rat cereb-ral cortex with cortical dysplasia（CD） by γ-ray irradiated injury.Methods γ-ray induced CD model rats were established and randomly divided into different groups according to postnatal age（neonatal rats,2 week,4 week,and adult rats）.The pathologic examination was performed,inclu-ding hematoxylin and eosin（HE）,immunohistochemistry and immunofluorescence.We observed the pathological change and the expression of uPAR in the rat cerebral cortex under a light micro-scope.Results Immunohistochemistry showed uPAR-positive cells in each group.Along with the growth of the age,positive cells gradually decrease,but the proportion of positive cells in CD model group is obviously higher than normal control.Double-labeled immunofluorescence stain-ing showed that uPAR-positive cells were mainly neurons.Conclusion In the process of CD,uPA-R may play an important role in the neural organization migration.Higher percentage of positive neurons is closely related with the process of epilepsy caused from CD.

关 键 词：皮质发育不良 癫痫 UPAR 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]