pAdKDR-tk/GCV自杀基因系统联合γ射线放疗鼻咽癌的体内实验  

In vivo experimental study on nasopharyngeal carcinoma with combination of pAdKDR-tk/GCV suicide gene therapysystem and ^(60)Co radiotherapy

作　　者：邱前辉[1,2] 孙伟[3] 陈少华[1,2] 黄晓明[3] 

机构地区：[1]广东省人民医院耳鼻咽喉头颈外科 [2]广东省医学科学院,广州510080 [3]中山大学第二附属医院耳鼻咽喉头颈外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2010年第9期414-416,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：广东省自然科学博士科研启动项目(No:5300821)

摘　　要：目的:探讨pAdKDR-tk/GCV自杀基因系统联合γ射线放疗人鼻咽癌的杀伤效应。方法:分别以pAdKDR-tk/GCV、60Coγ射线放疗及两者联合治疗裸鼠人鼻咽癌移植瘤模型,比较疗效;利用肿瘤生长曲线及肿瘤抑瘤率评价该自杀基因系统联合放疗的疗效。结果:单纯基因治疗与放疗对鼻咽癌CNE-2细胞裸鼠皮下移植瘤生长的抑瘤率分别为58.43%和70.88%,而基因治疗与放疗联合应用的抑瘤率达到84.39%,与前两者比较,差异有统计学意义(P<0.01);第21天基因治疗与放疗联合治疗组肿瘤平均体积仅为对照组的13.5%,明显低于单纯基因治疗与放疗。结论:pAdKDR-tk/GCV自杀基因系统联合放疗的疗效较单一治疗方案有显著的提高,为进一步开展靶向肿瘤血管内皮自杀基因联合放疗研究奠定了良好的理论和临床基础。Objective：To explore the killing effect of pAdKDR-tk/GCV suicide gene therapy system combined with 60Co radiotherapy on human nasopharyngeal carcinoma in vivo.Method：The pAdKDR-tk/GCV suicide gene therapy system and 60Co radiotherapy were used separately or in combination for human nasopharyngeal carcinoma in vivo to compare their effects. The tumor growth curve and inhibition rate of tumor to the cure effects of the combination of the pAdKDR-tk/GCV suicide gene therapy system and 60Co radiotherapy.Result：The inhibition rate of gene therapy alone and radiotherapy alone in curing the transplanted tumor in nude mouse subcutaneously was 58.43% and 70.88% respectively, and the combined application of gene therapy and radiotherapy exhibited stronger therapeutic effects （the inhibition rate was 84.39%,P0.01） ,the mean tumor volume of the combination group was only 13.5% of the mean tumor volume of compared group, and it was obviously lower than the mean tumor volume of gene therapy alone and radiotherapy alone in the twenty-first day.Conclusion：The combined application of gene therapy and radiotherapy has an obviously higher curative effect than simple therapy. This method would establish a theoretic and clinical basis for the research of combination of suicide gene system tumor vascular targeting Treating and radiotherapy.

关 键 词：单纯疱疹病毒-胸苷激酶 鼻咽肿瘤 自杀基因治疗 放射治疗 

分 类 号：R739.6[医药卫生—肿瘤]