大承气汤治疗大鼠实验性急性胰腺炎肠功能障碍的机制研究  

Effect of Da-Cheng-Qi decoction on enteric functional disturbance in rats with acute experimental pancreatitis and its mechanism

作　　者：薛育政[1] 刘宗良[1] 俞宪民[1] 陆宇峰[1] 戴泓[1] 尹群[1] 李建平[1] 

机构地区：[1]无锡市第三人民医院消化科,214041

出　　处：《中华消化杂志》2010年第4期259-262,共4页Chinese Journal of Digestion

摘　　要：目的探讨大承气汤治疗大鼠实验性急性胰腺炎（AP）肠功能障碍的可能机制。方法将72只成年SD大鼠随机分为假手术组（SO组）、胰腺炎组（AP组）和大承气汤治疗组，每组24只。经胆胰管内加压注射3．5％牛磺胆酸钠0．1ml／100g体重制备AP模型，治疗组在造模前给予大承气汤按2g／100g体重剂量灌胃1次，AP组和SO组则灌注等量0．9％氯化钠溶液。每组于造模后3、6、12h各处死8只大鼠并立即心脏取血，检测血清淀粉酶水平，酶联免疫吸附试验检测血清5羟色胺（5-HT）含量。同时取6h时间点各组大鼠距屈氏韧带10cm空肠、末端回肠及乙状结肠组织，采用RT—PCR和Western印迹法检测肠黏膜中5-HT3、5-HT4 mRNA及蛋白表达情况。结果①造模后AP组和治疗组血清淀粉酶、5-HT水平均明显高于SO组（P〈0．01），且每个时间点治疗组均较AP组显著降低（P％0．05）；AP组和治疗组5-HT水平均呈现出先升后降趋势。②与SO组相比，AP组5-HT3、5-HT4mRNA及蛋白表达水平在空肠、末端回肠及乙状结肠均明显下降（P〈0．01）；与AP组比较，治疗组各部位5-HT4mRNA及蛋白表达均增高（P〈0．05），而5-HT3mRNA及蛋白表达则未增加。结论5-HT升高及其受体降低可能是AP发生肠功能障碍的原因之一，而大承气汤治疗的主要作用可能是通过增加5HT。表达以改善肠功能，可作为AP肠功能障碍的治疗方法之一。Objective To investigate the effect of Da-Cheng-Qi decoction on enteric functional disturbance in rats with acute experimental pancreatitis and its potential mechanism. Methods Seventy-two SD rats were randomly, divided into sham operation group, acute pancrea＇titis （AP） group and Da-Cheng-Qi decoction treated group with 24 each. The AP model was induced by retrograde injection of sodium taurocholate （0.1 ml/100 g） into biliopancreatic duct. Before modeling, the rats in treatment group received Da-Cheng-Qi decoction （2 g/100 g）, and the rats in other two groups received 0.9% NaC1 solution. The rats were sacrificed at 3, 6 and 12 hours with 8 each, and the blood samples were taken for detecting the level of amylase and the contents of 5-hydroxytryptamine （5 HT） by enzyme-linked immunosorbent assay. The colonic tissues 10 cm apart from trans-ligament incluing jejunum, terminal ileum and sigmoid colon were collected for examining the expressions of 5-HT3 and 5-HT4mRNA and proteins by RT-PCR and Western blotting respectively. Results （D The levels of serum amylase and 5-HT in AP group and treatment group were significantly higher than those in sham operation group （P〈0.01）, but they were lower in treatment group than in AP group at each time points （P〈0. 05）. The level of 5-HT was increased in the initial stage and then decreased gradually both in AP group and treatment group. （2） The expressions of 5-HT3 and 5-HT4 mRNA and proteins were significantly decreased in the jejunum, terminal ileum and sigmoid colon in AP group compared with sham operation group （P〈0. 01）. Whereas the expressions of 5-HT3 mRNA and protein,but not 5-HT4, were increased in the treatment group in comparison with AP group （P 0.05）. Conclusions The level of 5-HT is significantly increased in acute pancreatitis, but its receptors （5-HT3 and 5-HT4 ） are decreased, which may induce enteric functional disturbance. The Da-Cheng- Qi decoction may improve enteric dynamic failure by increasing the expres

关 键 词：胰腺炎 5-羟色胺 肠功能障碍 大承气汤 

分 类 号：R576[医药卫生—消化系统]