氧化应激对小鼠NKG2D配体表达的影响  

Effects of oxidative stress on the expression of NKG2D ligands in mouse model

作　　者：李成红[1] 崔莲仙[1] 何维[1] 马驰[1] 

机构地区：[1]中国医学科学院基础医学研究所北京协和医学院基础学院免疫学系,北京100005

出　　处：《基础医学与临床》2010年第6期570-575,共6页Basic and Clinical Medicine

基　　金：国家高技术研究发展计划(863计划)(2006AA02Z432);国家自然基金(30872362;30490244)

摘　　要：目的分析氧化应激时小鼠免疫器官和免疫组织的NKG2D配体MULT1和Rae1表达的变化,研究氧化应激对免疫系统的影响。方法给小鼠吸入适量的臭氧,建立动物氧化应激模型;利用real-time PCR和免疫组织化学方法检测小鼠胸腺、脾脏、淋巴结和小肠MULT1和Rae1的表达。用流式细胞仪检测胸腺、脾脏的淋巴细胞和小肠上皮间淋巴细胞表面的MULT1和Rae1的表达。结果氧化应激组小鼠胸腺中MULT1表达降低而Rae1表达升高;脾脏的Rae1表达略有提高,MULT1的表达变化不明显;淋巴结中MULT1的表达变化不明显,Rae1表达降低;小肠的MULT1和Rae1表达均明显上调,多集中于小肠绒毛的上皮层。结论 MULT1和Rae1的表达在体内受到严格的调控。应激分子MULT1和Rae1可以作为检测体内应激状态的参考指标。Objective To analyze the effect of oxidative stress on the expression of NKG2D ligands MULT1 and Rae1in murin immune organs and tissues,and to study the effect on immune system.Methods We established an oxidative stress mice model through quantitative ozone inhalation.Then we detected the expression of MULT1 and Rae1 in mice thymus,spleen,lymph node and intestine by real-time PCR and immunohistochemistry after oxidative stress.The cell surface expressions on thymocytes,splenic lymphocytes and intestine intraepithelial lymphocytes（iIELs） were further examined by flow cytometry.Results In model mice the expression of MULT1 in thymus decreased and Rae1 increased;The expression of MULT1 in spleen had no obvious change and Rae1 increased;The expression of MULT1 in lymph node had no obvious change and Rae1 decreased;In small intestine both the MULT1 and Rae1 expression were up-regulated in epithelial layer.Conclusion There is more complicating regulating mechanism to control the expression of MULT1 and Rae1.MULT1 and Rae1 can be used as biological markers of stress.

关 键 词：氧化应激 Rae1 MULT1 NKG2D 表达 

分 类 号：R363[医药卫生—病理学]