血清瘦素与结直肠癌相关癌基因表达的相关性  

Correlation of serum leptin with oncogene expression in colorectal cancer

作　　者：黄哲[1] 沈名扬[1] 王俊江[1] 丛进春[1] 陈春生[1] 

机构地区：[1]中国医科大学附属盛京医院结直肠肿瘤外科,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2010年第14期1509-1513,共5页World Chinese Journal of Digestology

摘　　要：目的:研究血清瘦素与结直肠癌突变癌基因的相关性,探讨其参与结直肠癌发生发展的机制.方法:研究对象肿瘤组选择30例结直肠癌患者,选择24名健康成年人作为对照组.用双抗体夹心ABC-ELISA法测定血清的瘦素水平,采用RT-PCR方法测定肿瘤标本的癌基因K-ras以及抑癌基因P53、APC和DCC的表达,对各癌基因表达的差异与血清的瘦素水平做出相关性分析.结果:肿瘤APC抑癌基因表达阳性的患者血清瘦素水平要高于表达阴性者(3.78μg/L±1.85μg/Lvs2.41μg/L±2.53μg/L,P<0.05),且随着血清瘦素水平的升高APC基因的表达强度也逐渐增加(P<0.05),其余3种癌基因的表达与血清瘦素水平没有明显相关性(P>0.05).结论:瘦素参与结直肠癌发生发展的途径与抑癌基因APC的表达有关,与癌基因K-ras及抑癌基因P53、DCC的表达无关.AIM：To search possible pathways via which leptin participates in colorectal carcinogenesis by analyzing the correlation between serum leptin and oncogene expression.METHODS：Serum leptin and adiponectin levels were measured by avidin-biotin-peroxidase complex enzyme-linked immunosorbent assay（ABC-ELLSA） in 30 colorectal cancer patients and 24 normal controls.The expression of K-ras,P53,adenomatous polyposis coli（APC） and deleted in colorectal carcinoma（DCC） mRNAs in tumor samples was detected by reverse transcription-polymerase chain reaction（RT-PCR）.The correlation between oncogene expression and serum leptin and adiponectin was then analyzed.RESULTS：The level of serum leptin was significantly higher in APC-positive patients than in APC-negative ones（3.78 μg/L ± 1.85 μg/L vs 2.41 μg/L ± 2.53 μg/L,P 0.05）,and the relative expression level of APC mRNA increased with the increase in serum leptin levels（P 0.05）.No significant correlation was noted between serum leptin and the expression of K-ras,P53 and DCC mRNAs in colorectal cancer（all P0.05）.CONCLUSION：There is a significant correlation between serum leptin level and APC expression in colorectal cancer.No significant correlation is noted between serum leptin level and the expression of K-ras,P53 and DCC mRNAs in colorectal cancer.

关 键 词：结直肠癌 瘦素 癌基因 

分 类 号：R735.3[医药卫生—肿瘤]