NNK对BEP2D细胞耐药基因表达的影响  

Effect of NNK on the expression of the drug resistance gene in the BEP2D cells

作　　者：白雪丽[1] 程红霞[1] 黄海燕[2] 王英田[3] 

机构地区：[1]山东大学附属省立医院,济南250021 [2]山东省医学科学院 [3]山东大学附属千佛山医院

出　　处：《山东医药》2010年第21期15-17,共3页Shandong Medical Journal

基　　金：山东省自然科学基金项目(Q2007C11)

摘　　要：目的探讨4-甲基亚硝胺-1-3-呲啶基-1-丁酮(NNK)对BEP2D细胞多药耐药相关蛋白(MRP)、肺耐药相关蛋白(LRP)表达的影响。方法应用NNK 500μg/m l干预BEP2D细胞生长,收集连续干预后第10、20、30代NNK-500细胞,行接种裸鼠成瘤试验检验其成瘤性;采用RT-PCR检测BEP2D细胞,以及第10、20、30代NNK-500细胞的MRP、LRP表达量。结果经NNK干预后的第20、30代NNK-500细胞接种裸鼠成瘤,病理切片显示为高分化鳞癌;RT-PCR显示,在BEP2D细胞中MRP、LRP呈低度表达,第10、20代NNK-500细胞的表达量略有增加,第30代NNK-500细胞的表达量明显增加(P<0.01)。结论 NNK对BEP2D细胞有致癌性,可增加BEP2D细胞的MRP、LRP表达,降低化疗敏感性。Objective To explore the effect of 4-（metylnitro-samino）-1-（3-pyridyl）-1-butanone（NNK） on the expression of the multidrug resistance-associated protein（MRP）and the lung resistance protein（LRP） in the BEP2D cells.Methods The BEP2D cells were induced by 500 μg/ml NNK,and the 10th,20th and 30th generation of the cells were collected and injected into the nude mice,on the same time RT-PCR was applied to observe the expression of the MRP and LRP.Results The 20th and 30th generation had the ability to grow tumor in nude mice,and the tumor showed to be well-differentiated squamous-cell epithelioma by pathology.The expressions of MRP and LRP gene were low in the BEP2D cells.The expressions of MRP and LRP gene were increased in the 10th,20th generation of the cells,and the expressions of MRP and LRP gene were obviously increased in the 30th generation（P0.01）.Conclusion The BEP2D cells could transform induced by the NNK.The NNK could enhance the expression of the MRP,LRP gene in the 30th generation NNK-500 cells.The NNK could decrease the chemosensitivity of lung cancer cells.

关 键 词：肺肿瘤 4-甲基亚硝胺-1-3-呲啶基-1-丁酮 多药耐药相关蛋白 肺耐药相关蛋白 

分 类 号：R734.2[医药卫生—肿瘤]