危害马铃薯的茎线虫分离鉴定  

Identification of Ditylenchus destructor from potatoes in Zhangjiakou,Hebei

作　　者：郭全新[1] 简恒[1] 

机构地区：[1]中国农业大学植物病理系,北京100193

出　　处：《植物保护》2010年第3期117-120,共4页Plant Protection

基　　金：公益性行业科研专项(NYHYZX07-050);农业部"948"项目(2006-G54);国家"十一五"科技支撑计划项目(2006BAD08A08)

摘　　要：本文对采自河北张家口市察北区、危害马铃薯的线虫(编号De-Chabei)进行了形态及分子生物学鉴定,形态测量结果表明其与马铃薯腐烂茎线虫(Ditylenchus destructor Thorne)一致,采用通用引物rDNA1/rDNA2扩增rD-NA-ITS区的长度为1130bp,测序比对以及采用马铃薯茎线虫B型特异性引物DdL1/DdL2扩增得到485bp特异性片段,表明与B型或L型马铃薯腐烂茎线虫一致;再次证明该型线虫在河北马铃薯主产区的分布和危害。A nematode population, named De-Chabei, was isolated from potato in the Chabei District of Zhangjiakou City, Hebei Province. It had been identified as type B or L of Ditylenchus destructor Thorne based on morphological and molecular methods. The amplified products of rDNA-ITS regions with the universal primers rDNA1/rDNA2 and the specific primers DdL1/DdL2 were 1 130 bp （GenBank accession No. FJ911551） and 485 bp, respectively. The results revealed that this type of D. destructor has existed in the potato-growing areas of Hebei province.

关 键 词：马铃薯 马铃薯腐烂茎线虫 形态鉴定 ITS区分析 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]