乌梁素海可培养细菌丰度和多样性分析  

BACTERIAL ABUNDANCE AND DIVERSITY IN LAKE WULIANGSUHAI AS REVEALED BY CULTURE METHOD

作　　者：赵春霞[1] 刘惠荣[1] 冯福应[1] 何一平[1] 

机构地区：[1]内蒙古农业大学生命科学学院应用与环境微生物实验所,呼和浩特010018

出　　处：《内蒙古农业大学学报（自然科学版）》2010年第2期193-197,共5页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金项目(30760004);内蒙古自治区自然科学基金项目(200711020304);内蒙古自治区高等学校科学研究项目(NJ06106);内蒙古农业大学博士科研启动基金(BJ06-50).

摘　　要：在水生生态系统中,细菌是复杂有机物和矿质元素转化的最重要的贡献者,丰度和多样性是了解其作用和功能、并有效利用的前提和关键。本文通过对乌梁素海富营养化湖区的可培养浮游细菌数量统计和多样性进行分析,以期揭示可培养浮游细菌的丰度和多样性,探讨他们与乌梁素海富营养化之间的关系。研究结果表明,乌梁素海水体中浮游细菌总数为0.1×10^6-0.6×10^7cfu/L。乌梁素海富营养化湖区可培养浮游细菌划线培养获得的41菌株,分属于27个分类单元,与数据库中已知的芽孢杆菌属（Bacillus）、类芽孢杆菌属（Paenibacillus）、微球菌属（Mi-crococcus）、杆状菌（brevundimonas）、苯丙酮酸莫拉氏菌（Marinibacillus）、假单胞菌属（Pseudomonas）细菌和动性球菌属（Planococcus）具有很高的相似性,γ-proteobacteria中的某些类群成为优势菌群可能是对乌梁素海水体富营养化的响应,为今后开发利用乌梁素海生物资源和修复乌梁素海生态环境提供了依据。In aquatic ecosystems,Bacteria is a complex transformation of organic matter and the most important in contributor to mineral elements.It is particularly important to comprehensive understand of abundance and diversity of aquatic ecosystems.In this paper,aimed to reveal the bacterioplankton abundance and diversity and understand how they related to eutrophication,bacterial diversity and numbers of Wuliangsuhai were analyzed.The results showed that the total number of bacteria of Lake Wuliangsuhai water is 0.1×10^6-6.0×10^6 CFU/L.Isolations of 41 strains were obtained and their 16S rDNA were sequenced and analyzed.Classification based on 16S rDNA revealed that all isolated bacteria fell into 27 OTU,among which they were highly similar with known Bacillus（Bacillus）,such as Paenibacillus,Micrococcus,brevundimonas,Marinibacillus Pseudomonas Planococcus,espectively.This may contribute for conservation and utilization of microbial resources as well as for restoration of ecological environment of Lake Wuliangsuhai.

关 键 词：乌梁素海 可培养细菌 微生物多样性 16S RDNA 

分 类 号：S917.1[农业科学—水产科学]