HLA-Ⅰ、CD_(68)和CD_3抗原在胃癌及周围淋巴结的表达及意义  

The expression and significance of HLA-Ⅰ CD_(68) and CD_3 in gastric carcinoma and lymph node

作　　者：张国建[1] 吴爱须[1] 闫庆辉[1] 王凤安[1] 蔡建辉[1] 刘津[1] 段国强[1] 

机构地区：[1]河北医科大学第二医院胃肠外科,石家庄市050000

出　　处：《河北医药》2010年第9期1049-1050,共2页Hebei Medical Journal

基　　金：河北省医学科学研究重点课题(编号:05232)

摘　　要：目的探讨HLA-Ⅰ、CD68和CD3抗原在胃癌及周围淋巴结的表达。方法胃癌组织30例,胃重度不典型增生组织30例做对照。周围淋巴结共40个。用抗HLA-Ⅰ、CD68和CD3的单克隆抗体采用SP法做免疫组化分析。结果阳性组织中细胞膜和细胞浆着色。HLA-Ⅰ抗原阳性表达率胃癌组织为16.67%,胃不典型增生组织为40%,二者差异有统计学意义(P<0.05);CD68阳性表达率为36.67%,胃不典型增生组织为26.67%,二者差异无统计学意义(P>0.05);CD3阳性表达率为20%,胃不典型增生组织为36.67%,二者差异无统计学意义(P>0.05)。淋巴结CD68阳性表达率为20%,CD3阳性表达率为35%。HLA-Ⅰ和CD68、CD3三者表达呈直线相关关系。结论胃癌HLA-Ⅰ表达下降,HLA-Ⅰ和CD68、CD3浸润有相关关系。Objective To investigate the protein expression of HLA-I, CD68 and CD3in gastric carcinoma and lymph node. Methods 30 cases of gastric carcinoma tissue,30 cases of gastrie severe dysplasia and 40 lymph nodes were enrolled in the study. The expression of HLA-I, CD68 and CD3 was detected by immunohistochmical staining method. Results The expression of HLA-I, CD68 and CD3on cell membrane and cytoplasmic expression in positive tissues were found. The positive expression of HLA-I was observed in 16.67% gastric carcinoma cases and 40% gastric severe dysplasia, and there was a significant difference between the two kinds of tissues （ P 〈 0.05 ）. The positive expressions of CD6swere observed in 36.67% gastric carcinoma cases and 26.67% gastric severe dysplasia, and there was no significant differenee between the two kinds of tissues （P 〉 0. 05） . The positive expressions of CD3 were observed in 20% gastric carcinoma cases and 36.67% gastric severe dysplasia, and there was no significant difference between the two kinds of tissues（ P 〉0.05）. The expressions of CD68 and CD3 were found in 20% and 35% lymph node. The expression of HLA-I, CD6s and CD3 had linear correlation with each other. Conclusion The expression of HLA-Ion gastric carcinoma decreases. The expression of HLA-I, CD6s and CD3 has a linear correlation with each other in gastric carcinoma.

关 键 词：胃癌HLA-Ⅰ CD68 CD3 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]