黄芪多糖对奶牛乳房炎主要致病菌的体外抑菌作用  

Antibacterial Effects of Astragalus Polysaccharides on Main Pathogenic Bacteria Causing Dairy Cow Mastitis in Vitro

作　　者：杨玉艾[1] 杨林富[1] 杨亮宇[1] 初晓辉[1] 毕保良[1] 孙永科[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《动物医学进展》2010年第6期47-49,共3页Progress In Veterinary Medicine

基　　金：云南省教育厅项目(08C0072);云南省科技厅项目(2008CD134;2009CD063);高层次科技人才培引工程项目(2009CI125-10;2009CI125-14)

摘　　要：采用水提醇治法提取黄芪多糖,并应用琼脂扩散法研究了黄芪多糖对几种奶牛乳房炎主要致病菌的抑制效果。结果表明,10 mg/mL的黄芪多糖对3种细菌都有一定的抑制效果,其中对大肠埃希菌的抑制作用最强,抑菌圈达11.05 mm±0.31 mm,对链球菌和金黄色葡萄球菌的抑菌圈分别为8.50 mm±0.71 mm和9.32 mm±0.21 mm。3种细菌的最小抑菌浓度不同,对大肠埃希菌的最小抑菌浓度为6.6 mg/mL,而对金黄色葡萄球菌和链球菌则为10.0 mg/mL。Astragalus polysaccharides（APS） were extracted from astragalus membran aceus after ethanol extraction by the method of circumfluence urine water as solvent, for studying it＇s antibacterial effects on pathogenic bacteria of dairy cow in vitro through agar diffusion method. The results of the antimicrobial activity of APS showed that 10 mg/mL APS could significantly inhibit the proliferation of three strains, and the inhibition E. coti was stronger than that to others with the diameter of inhibition zone reached 11.05 mm±0. 31 mm. The diameter of inhibition zone against Streptococcus and Staphylococcus aureus were 8.50 mm±0.71 mm and 9.32 mm±0.21 ram,respectively. The minimal inhibitory concentrations to three strains were different, which were 6. 6 mg/mL to E. coli, and 10. 0 mg/mL to Streptococcus and Staphylococcus aureus.

关 键 词：黄芪多糖 奶牛 乳房炎 致病菌 抑菌 

分 类 号：S853.74[农业科学—临床兽医学]