HBsAg HBeAg作用PBLs对TCR Vβ基因表达的影响  

Effects of HBsAg and HBeAg on TCR Vβ gene expressions in peripheral blood lymphocytes *

作　　者：黄树林[1] 陈红清[1] 李菁华 李依娜[1] 罗利琼[1] 

机构地区：[1]广东药学院分子生物学研究室

出　　处：《世界华人消化杂志》1999年第1期25-27,共3页World Chinese Journal of Digestology

基　　金：国家自然科学基金

摘　　要：目的探讨乙肝病毒（ＨＢＶ）与肝癌的相关性．方法取用乙肝疫苗注射３次前后外周血淋巴细胞（ＰＢＬ）；ＨＢＶＤＮＡ转染的ＨｅｐＧ２２２１５株培养上清（ＨＢｓＡｇ，ＨＢｅＡｇ阳性）感染健康人ＰＢＬ后进行ＴＣＲＶβ基因１－２０亚家族表达水平的分析．结果乙肝疫苗注射后及ＨｅｐＧ２２２１５株培养上清感染健康人ＰＢＬ后ＴＣＲＶβ６，１４；Ｖβ６，１５特异性扩增而表达水平显著增高．结论Ｖβ６可能为识别ＨＢＶ抗原或引发免疫应答的基因片段，但Ｖβ１４，１５各自在限制和杀伤功能方面起着重要的作用。AIM To investigate the relationship between hepatitis B virus (HBV) and hepatocellular carcinoma. METHODS The TCR Vβ1-20 gene repertoire was analysed by reverse transcription polymerase chain reaction (RT PCR) in peripheral blood lymphocytes (PBL) from individuals vaccinated with recombinant hepatitis B vaccine, PBLs from healthy donors stimulated by supernatants (HBsAg and HBeAg positive) of the HepG2 2215 cell line which was transfected by HBV DNA. RESULTS The specific amplifications of Vβ6, 14 and Vβ6,15 were observed in PBL after vaccination and stimulated by HepG2 cell line respectively. CONCLUSION The Vβ6 segments may be specific recognitive units for HBV antigens or a trigger of the immune response, and Vβ14, 15 night play an important role in restriction and cytotoxicity. The relation between HBV and hepatocellular carcinoma was demonstrated at molecular level.

关 键 词：乙型肝炎病毒 肝肿瘤 HBSAG HBEAG 基因表达 

分 类 号：R735.702[医药卫生—肿瘤]