脂蛋白、钙拮抗剂对大鼠贮脂细胞的影响  

Effect of low density lipoprotein and calcium antigonists on fat storing cells in rat

作　　者：王炯[1] 李定国[1] 陆汉明[1] 孙志广[1] 蒋祖民 徐芹芳[1] 顾鹤定[1] 陈颖伟[1] 

机构地区：[1]上海第二医科大学附属新华医院

出　　处：《世界华人消化杂志》1999年第1期57-59,共3页World Chinese Journal of Digestology

摘　　要：目的观察脂蛋白、钙拮抗剂对大鼠贮脂细胞增殖、透明质酸及胶原合成的影响．方法通过ＭＴＴ法，透明质酸放射免疫分析法及３Ｈ脯氨酸掺入法测定了低密度脂蛋白（ｌｏｗｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ，ＬＤＬ）、钙拮抗剂尼卡地平（Ｎｉｃａｒｄｉｐｉｎｅ，Ｎｉｃ）和维拉帕米（Ｖｅｒａｐａｍｉｌ，Ｖｅｒ）及其联合应用对大鼠贮脂细胞（ｆａｔｓｔｏｒｉｎｇｃｅｌ，Ｆｓｃ）增殖、透明质酸（ｈｙａｌｕｒｏｎｉｃ，ＨＡ）及胶原合成的影响．结果ＬＤＬ能促进ＦＳＣ增殖、ＨＡ及胶原合成，同时Ｎｉｃ及Ｖｅｒ对ＬＤＬ刺激引起的ＦＳＣ增殖、胶原合成有明显的抑制作用，但对ＨＡ的抑制作用不明显．结论ＬＤＬ参与ＦＳＣ的增殖及细胞外基质的合成。AIM To observe the effect of low density lipoprotein and calcium antagonists on the proliferation, HA and collagen synthesis of fat storing cells (FSCs). METHODS In this study, the effect of low density lipoprotein (LDL) and calcium antafonists Nicardipine (Nic) and Verapamil (Ver) and the combination of them on the proliferation, HA and collagen synthesis of fat storing cells (FSCs) were determined by MTT colorimetric assay, hyaluronic acid radioimmunoassay and 3 H proline incorporation assay were used to determine the effects of above factors. RESULTS LDL could accelerate the proliferation, HA and collagen synthesis of FSCs. Nic and Ver could inhibit the proliferation and collagen synthesis of FSCs. Ver also inhibited the HA synthesis. Meanwhile, Nic and Ver had marked inhibitive effects on proliferation and collagen synthesis of FSCs stimulated by LDL, but the inhibition on synthesis of HA was not obvious. CONCLUSION LDL is involved in the proliferation and synthesis of HA and collagen of FSCs. Nic and Ver had resistant effects on LDL.

关 键 词：肝硬化 脂蛋白 尼卡地平 维拉帕米 贮脂细胞 

分 类 号：R575.205[医药卫生—消化系统]