二胺氧化酶在急性坏死性胰腺炎肠道损伤中的作用  

Effect of DAO on intestinal damage in acute necrotizing pancreatitis in dogs

作　　者：吴承堂[1,2] 黎沾良[1,2] 

机构地区：[1]第一军医大学南方医院普外科 [2]中国人民解放军304医院普外科

出　　处：《世界华人消化杂志》1999年第1期64-65,共2页World Chinese Journal of Digestology

摘　　要：目的探讨急性坏死性胰腺炎（ＡＮＰ）时血浆二胺氧化酶（ＤＡＯ）水平变化及其与肠道损伤的关系．方法通过向主胰管内注入牛磺胆酸钠和胰蛋白酶复制犬ＡＮＰ模型（ｎ＝８）；检测血清淀粉酶水平；测定血浆、肠组织ＤＡＯ活性；测量回肠粘膜绒毛形态学改变．结果ＡＮＰ发病后ｄ１（ｍｏｌ／ｓ，００２５±０００６ｖｓ００１１±０００２），ｄ２（ｍｏｌ／ｓ，００１９±０００５ｖｓ００１２±０００３）血浆ＤＡＯ活性增加，ｄ４（ｍｏｌ／ｓ，０００６±０００１ｖｓ００１４±０００２）以后明显下降，肠组织ＤＡＯ活性亦显著下降；回肠粘膜绒毛萎缩、脱落，高度、宽度降低，面积减少；血清淀粉酶水平升高３～１０倍；肝、胰、脾、肺、肾及肠系膜淋巴结出现细菌移位．结论ＡＮＰ时血中ＤＡＯ活性变化可反映肠粘膜上皮损伤。AIM To investigate the relationship between plasmic diamine oxidase (DAO) and gut injury in acute necrotizing pancreatitis (ANP). METHODS ANP was induced by injection of sodium taurocholate with trypsin into the pancreatic duct in 8 dogs during abdominal operation. Serum levels of amylase was determined, DAO activities of plasma and ileal mucosa were measured dynamically. RESULTS In ANP group, the levels of plasmic DAO increased significantly in the first ( mol/s ,0 025±0 006 vs 0 011±0 002) and the second day ( mol/s ,0 019±0 005 vs 0 012±0 003), but after the fourth day ( mol/s ,0 006±0 001 vs 0 014±0 002) it decreased. The levels of DAO in ileum were also lowered. Serum levels of amylase was increased 3-10 times. ANP induced damage of epithlial villi of the mucosa and bacterial translocation was found in liver, pancreas, kidney, lung, spleen and mesenteric lymph nodes of all animals. CONCLUSION Plasma DAO level might reflect the damage of intestinal mucosa in ANP, and bacterial translocation is the result of the gut injury.

关 键 词：胰腺炎 组胺酶 肠道损伤 病理学 

分 类 号：R576.06[医药卫生—消化系统] R574.02[医药卫生—内科学]