大肠菌群荧光定量PCR方法和多管发酵法检测结果相关性研究  

THE CORRELATION STUDY OF COLIFORMS DETECTION IN WATER BETWEEN FLUORESCENCE QUANTITATIVE PCR AND MULTIPLE TUBE FERMENTATION METHOD

作　　者：林启辉 刘渠[2] 李涌[2] 苏雪媚[3] 

机构地区：[1]深圳市观谰预防保健所,深圳518110 [2]深圳市龙岗区疾病预防控制中心 [3]深圳市龙岗区人民医院

出　　处：《现代预防医学》2010年第11期2081-2083,共3页Modern Preventive Medicine

摘　　要：[目的]比较大肠菌群荧光定量PCR方法和多管发酵法检测结果的相关性。[方法]采用建立的大肠菌群荧光定量PCR方法和多管发酵法同时检测系列浓度梯度的实验室模拟水样的大肠菌群含量。对结果进行相关性分析,比较两种方法定量结果的相关性。[结果]大肠菌群荧光定量PCR能够能准确检测高于102cfu/100ml水平的水质大肠菌群含量,在此水平以上的定量结果同多管发酵法结果具有较高相关性。[结论]大肠菌群荧光定量PCR方法具有快速、特异、操作简便的特点,可以作为多管发酵法检测方法的补充,用于水质大肠菌群的快速定量检测。[Objective] To study the correlation between quantitative PCR and multiple tube fermentation method of coliform detection. [Methods] A series of simulated water samples were detected by fluorescence quantitative PCR established and multiple tube fermentation method simultaneously, the results were analysis to find the correlation between the two methods. [Results] The fluorescence quantitative PCR of coliform could quantitatively detect water samples which contained coliform higher than 102cfu/100ml. The result was significantly correlated with the results of multiple fermentation method. [Conclusion] The fluorescence quantitative PCR method is a rapid, specific and convenient detection method of coliform, which could be used as a complement of traditional method and applied to rapid quantitative detection in water.

关 键 词：大肠菌群 荧光定量PCR 多管发酵法 相关性 

分 类 号：R115[医药卫生—公共卫生与预防医学]