硅胶柱层析法分离生姜精油中的β-水芹烯  

Separation of β-Phellandrene from Ginger Essential Oil by Silica Gel Column Chromatography

作　　者：薛岩明 杜爱玲[1] 贾雁高[1] 杜爱琴[1] 

机构地区：[1]山东大学化学与化工学院,山东济南250061

出　　处：《精细化工》2010年第5期433-436,441,共5页Fine Chemicals

基　　金：山东省科技发展计划项目(2007GG30009010)~~

摘　　要：利用硅胶柱层析对生姜精油中的β-水芹烯进行分离研究,采用紫外分光光度法(UV)、气相色谱-质谱联用(GC-MS)和高效液相色谱(HPLC)对分离样品进行分析,结果表明,柱层析适宜条件为:正己烷为洗脱剂,m(生姜精油)∶m(硅胶)=1∶50,洗脱剂流速为2.0 mL/min。在该条件下,经过两次硅胶柱层析,可得到β-水芹烯的峰面积百分数为95.18%的样品。The separation of β-phellandrene from ginger essential oil by silica gel column chromatography was investigated.The UV spectrophotometry and gas chromatography-mass spectrometry（GC-MS） and high-performance liquid chromatography（HPLC） were used to analyze the separated samples.The results showed that the column chromatography was preferably conducted under such conditions：n-Hexane as eluant,m（ginger essential oil）∶m（silica gel）=1∶50,and the eluant flow rate of 2.0 mL/min.Under these operational conditions and after twice silica gel column chromatography,the GC-MS peak area percentage of 95.18% for the sample of β-phellandrene can be obtained.

关 键 词：Β-水芹烯 生姜精油 硅胶柱层析 UV GC-MS 分离提纯 

分 类 号：TQ028[化学工程] R284.2[医药卫生—中药学]