电刺激三叉神经节对蝶腭神经节内一氧化碳合酶和血管活性肠肽阳性神经元的影响  

Effects of Trigeminal Ganglion Electrical Stimulation on the Positive Neurons of Nitric Oxide Synthase and Vasoactive Intestinal Polypeptide in Sphenopalatine Ganglion of Migraine Rats

作　　者：曹月洲[1] 徐武[1] 韦俊超[1] 万琪[1] 

机构地区：[1]南京医科大学附属第一医院神经内科,210029

出　　处：《中国临床神经科学》2010年第3期269-272,共4页Chinese Journal of Clinical Neurosciences

摘　　要：目的:观察实验性偏头痛大鼠副交感神经蝶腭神经节内一氧化氮合酶(NOS)和血管活性肠肽(VIP)阳性神经元的变化。方法:12只雄性SD大鼠随机分为实验组和对照组(均n=6)。实验组为电刺激三叉神经节建立的偏头痛大鼠模型。对照组仅作手术而不刺激三叉神经节。用组织化学的方法观察蝶腭神经节内NOS阳性神经元的变化,用免疫荧光法观察蝶腭神经节内VIP阳性神经元的变化。结果:实验组和对照组大鼠的蝶腭神经节内均有NOS和VIP阳性神经元,但实验组NOS和VIP阳性神经元均较对照组显著增加(P<0.01)。结论:电刺激三叉神经节可以通过三叉-副交感神经反射系统,显著升高蝶腭神经节中NOS和VIP神经元的数目。偏头痛发病过程中脑膜及颅内大血管的剧烈扩张很可能与蝶腭神经节中NOS和VIP神经元增加有关。Aim： To investigate the changes of the positive neurons of nitric oxide synthase（NOS） and vasoactive intestinal polypeptide（VIP） in sphenopalatine ganglion in migraine model of rat. Methods： 12 male SD rats were divided randomly into the experimental group and the control group （n=6 in each group）. The experimental group was termed as migraine model and the rats were treated by electrical stimulating trigeminal ganglion, while the rats of the control group were only operated without electrical stimulating. The positive neurons of NOS and VIP were detected in sphenopalatine ganglion by using histochemical and immunoflurescence histochemical staining, respectively. Results： The numbers of both NOS and VIP positive neurons in sphenopalatine ganglion in experimental group were increased significantly compared with those in the control group（P〈0.01）. Conclusion： The trigeminal autonomic reflex may be actived by lligeminal ganglion electrical stimulation, which then significantly increased the numbers of both NOS and VIP positive neurons in sphenopalatine ganglion. Violent dilation of meningeal vessels and intracranial large vessels maybe involved in the increased numbers of both NOS and VIP positive neurons in sphenopalatine ganglion in pathogenesis of migraine.

关 键 词：偏头痛 一氧化氮合酶 血管活性肠肽 蝶腭神经节 

分 类 号：R747.2[医药卫生—神经病学与精神病学]