高羊茅FaChit1基因cDNA克隆及诱导表达  

Cloning and Inducible Expression of a Class Ⅰ Chitinase Gene FaChit1 from Festuca arundinacea

作　　者：王健[1,2] 徐子勤[2] 

机构地区：[1]安康学院农学与生命科学学院,陕西安康725000 [2]西北大学西部资源和生物技术教育部重点实验室,西安710069

出　　处：《西北植物学报》2010年第5期869-875,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30870194);陕西省自然科学基金(2006C103)

摘　　要：应用简并RT-PCR及RACE技术,从高羊茅中克隆了1个Ⅰ类几丁质酶基因cDNA全长序列,命名为Fa-Chit1.结果表明,该cDNA具有1个951 bp的完整编码框,编码316个氨基酸,其编码产物和其它植物的Ⅰ类几丁质酶在氨基酸序列上具有较高的同源性,包含典型的几丁质结合区、催化区以及脯氨酸、半胱氨酸富集的铰链区,但缺少定位到植物液泡所必须的C末端延伸区靶向信号.Northern杂交显示,FaChit1对真菌激发子有较强的响应,乙烯和干旱胁迫均能有效诱导FaChit1基因的表达,而对机械损伤处理的反应比较微弱,只在叶片中积累少量的mRNA.The full-length cDNA sequence of FaChit1,a class Ⅰ chitinase gene from Festuca arundinacea,was isolated by the combination of degenerate RT-PCR,RACE and characterized subsequently.The results showed that the full-length open reading frame contains 951 base pairs and contains a deduced class Ⅰ chitinase containing 316 amino acids,which was consistent with the properties of most previously published plant class Ⅰ chitinases.The deduced amino acid sequence of FaChit1 contains the chitin binding,catalytic,and proline and glycine-rich domains characteristic for most class Ⅰ chitinases,but no C-terminal extension region.Northern blotting revealed that FaChit1 is induced effectively by fungal elicitors,dehydration,and ethylene,but only slightly by mechanical wounding in leaf.

关 键 词：高羊茅 Ⅰ类几丁质酶基因 FaChit1 胁迫诱导 

分 类 号：Q786[生物学—分子生物学]