Mrh-aFGF对帕金森病大鼠黑质神经元的保护  

Protective effect of modified recombinant hunan aFGF on the neurons in substantia nigra of Parkinson disease rats

作　　者：肖春苟[1] 沈伟哉[2] 郭国庆[2] 李校堃[3] 

机构地区：[1]韶关学院医学院解剖教研室,广东韶关512026 [2]暨南大学医学院解剖教研室,广州510630 [3]温州医学院生物与天然药物研究院,浙江温州325027

出　　处：《中国临床解剖学杂志》2010年第3期312-316,共5页Chinese Journal of Clinical Anatomy

基　　金：863国家基金(2001AA215131)

摘　　要：目的探讨重组人改构体酸性成纤维细胞生长因子(Mrh-aFGF)对帕金森病(PD)大鼠黑质神经元病变的影响。方法 SD大鼠72只,随机分为4组:对照组、PD组、生理盐水(NS)处理组、Mrh-aFGF处理组,每组18只。6-OHDA分别注入左侧黑质和腹侧被盖区后建立PD大鼠模型,侧脑室内注射Mrh-aFGF,用阿扑吗啡诱导旋转行为;Nissl染色法观察大鼠黑质神经元病理学改变;电子显微镜观察黑质神经元超微结构的变化。结果对照组均未出现旋转行为;PD组术后旋转速度逐渐加快;NS处理组旋转行为未见明显改善;Mrh-aFGF处理组旋转速度明显减慢(P<0.05);PD组及NS处理组大鼠损毁侧黑质神经元数目较健侧显著减少(P<0.05);Mrh-aFGF处理组术后1周、2周、4周损毁侧神经元数目均较PD组及NS处理组明显增加(P<0.05);PD组大鼠黑质神经元超微结构明显受损,出现核固缩,线粒体肿胀、嵴消失,粗面内质网扩张、脱颗粒,以及突触前后膜肿胀,突触间隙消失,Mrh-aFGF处理组黑质神经元超微结构有明显改善。结论 Mrh-aFGF能改善PD大鼠的旋转行为,减少PD大鼠黑质神经元的丢失,并改善其黑质神经元的超微结构。Objective To explore the protective effect of modified recombinant hunana FGF（Mrh-aFGF） on the neurons in substantia nigra of Parkinson disease rats.Methods The Parkinson＇s disease（PD） rats were induced by injecting 6-OHDA into the left substantia nigra compacta（SNC） and ventral tegmental area（VTA）,Then,the PD rats were treated with Mrh-aFGF or saline by lateral ventricle injection.The behavioral changes of the treated rats were detected after apomorphine injection.The morphologic features of the neurons in the substantia nigra were observed by Nissl＇s staining and electronic microscopy.Results Rotation behavior was not found in the intacted rats,otherwise,the average rotation rate speeded by degrees in PD and NS groups,however,rats treated with Mrh-aFGF showed slower rotation rate（P0.05）.In PD and NS groups,the neurons in left（the injured side）SNC of PD rats decreased significantly（P0.05） compared to that of right side.In Mrh-aFGF group,the structure of the left（the injured side）SNC was significantly improved and the number of neurons was increased,compared with that of PD and NS groups（P0.05）.The nigral neurons of PD rats appeared karyopyknosis,the swollen mitochondria,and the crista lost,as well,endoplasmic reticulum was found to be dilatated and degranulated obviouly,moreover,pre-synaptic and post-synaptic membranes were not clear and the synaptic space disappeared.In Mrh-aFGF group,the ultrastructure of nigral neurons,such as nuclei,mitochondria,synaptic structure,kept well compared to that of PD group.Conclusions Mrh-aFGF could modify the rotation behavior,protect nigral neurons from the loss and keep the normal ultrastructures of nigral neurons of Parkinson disease rats.

关 键 词：帕金森病 Mrh-aFGF 黑质 超微结构 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]