凋亡抑制蛋白Livin在胃癌及癌旁组织中的表达  

Expression of inhibitor of apoptosis protein Livin in gastric cancer and adjacent non-cancerous tissue

作　　者：侯毅鞠[1] 李艳[1] 袁忠海[1] 郭素红[1] 

机构地区：[1]吉林医药学院临床检验系,吉林省吉林市132013

出　　处：《世界华人消化杂志》2010年第12期1196-1200,共5页World Chinese Journal of Digestology

基　　金：吉林省教育厅"十一五"基金资助项目;No.2008405~~

摘　　要：目的:分析Livin蛋白在胃癌及癌旁组织中的表达,并根据其与胃癌生物学行为之间的关系分析对胃癌预后评估的意义.方法:采用免疫组织化学SP法检测50例胃癌及癌旁组织中Livin蛋白,以Image-ProPlus6.0专业图像分析软件定量分析其表达.并通过比较不同类型组织切片中表达量的差异,分析其在胃癌中表达的意义.结果:Livin蛋白在胃癌组织中表达显著高于癌旁组织(IA:283270.55±199604.16vs6878.08±9214.56,P<0.01).不同类型癌组织中表达亦有显著差异,高-中分化癌组织明显低于低-未分化(41288.94±27891.09vs359690.5±166583.17,P<0.01);浸润未达浆膜层(T1-2)者显著低于达浆膜层(T3-4)者(85529.62±115287.42vs360174.77±170507.09,P<0.01);伴淋巴转移者显著高于无淋巴转移(380949.72±171073.92vs148547.51±152479.47,P<0.01).结论:Livin蛋白高表达于胃癌组织,在不同分化程度、浸润度以及有无淋巴转移组间的表达都有显著差异.Livin蛋白高度表达预示肿瘤有较高的侵袭性,可用于判断胃癌预后.AIM：To detect the expression of Livin protein in gastric cancer and adjacent non-cancerous tissue,and to analyze its prognostic significance by exploring the relationship between Livin expression and the biological behavior of gastric cancer.METHODS：The expression of Livin was detected by immunohistochemistry （streptavidinperoxidase method） in 50 gastric cancer and matched adjacent non-cancerous tissue samples.Quantitative analysis of Livin expression was performed with the Image-Pro Plus 6.0 software.The significance of Livin expression in gastric cancer was then analyzed.RESULTS：The integrated absorbance （IA） value of Livin staining in gastric cancer tissue was significantly higher than that in adjacent non-cancerous tissue （283 270.55 ± 199 604.16 vs 6 878.08 ± 9 214.56,P 〈0.01）.The IA value was lower in well and moderately differentiated cancer tissue than in poorly and undifferentiated tissue （41 288.94 ± 27 891.09 vs 359 690.5 ± 166 583.17,P M0.01）,in cancer tissue not invading the serosal layer than in that invading the serosal layer （T3-4） （85 529.62 ± 115 287.42 vs 360 174.77 ± 170 507.09,P〈 0.01）,and in gastric cancer without lymph node metastasis than in that with lymph node metastasis （148 547.51 ± 152 479.47 vs 380 949.72 ± 171 073.92,P〈 0.01）.CONCLUSION：Livin protein is highly expressed in gastric cancer.Livin expression is significantly associated with tumor differentiation,infiltration and lymph node metastasis in gastric caner.Livin protein may be used as a prognostic marker for gastric cancer.

关 键 词：胃癌 LIVIN 诊断 预后 

分 类 号：R735.2[医药卫生—肿瘤]