顺铂结合CIK细胞对裸鼠人胃癌模型体内的抗肿瘤作用  

Antitumor effects of cisplatin and CIK cells against human gastric cancer in nude mice

作　　者：孙利兵[1] 杨光[1] 

机构地区：[1]南京医科大学附属苏州医院肿瘤内科,江苏省苏州市215001

出　　处：《世界华人消化杂志》2010年第12期1261-1263,共3页World Chinese Journal of Digestology

基　　金：苏州科技局基金资助项目;No.SS0524~~

摘　　要：目的:探讨顺铂、CIK细胞以及顺铂联合CIK对裸鼠人胃癌移植瘤生长的抑制作用,为临床上联合应用顺铂和CIK细胞治疗胃癌提供实验依据.方法:应用淋巴细胞分离液分离外周血单个核细胞,给予多种细胞因子(rhIFN-g、CD3mcAb、rhlL-1、rhlL-2),诱导生成CIK细胞;培养人胃癌细胞株SGC-7901,接种至80只裸鼠右腋皮下,10d后取移植瘤直径基本一致的裸鼠随机分4组:NS对照组、顺铂组、CIK细胞组和顺铂+CIK细胞组,每组16只.连续5d在接种肿瘤细胞部位处给予相应注射治疗,观察其对胃癌移植瘤模型的抗肿瘤疗效.结果:与NS对照组相比,顺铂组、CIK细胞组、顺铂+CIK细胞组裸鼠人胃癌移植瘤模型治疗后肿块质量均减轻,生存期均明显延长,尤以顺铂+CIK组效果显著(P<0.01).裸鼠体内实验表明,顺铂+CIK细胞联合应用能够显著抑制胃癌细胞的生长,其抑瘤率可达57.8%,明显高于NS对照组(P<0.01).免疫功能检测显示红细胞C3b反应受体明显升高,红细胞免疫复合物受体明显降低(P<0.01).结论:顺铂联合CIK细胞对人胃癌移植瘤生长的抑制作用大于单独应用顺铂或CIK细胞.AIM：To investigate the antitumor effects of cisplatin and cytokine-induced killer （CIK） cells,alone and in combination,against human gastric cancer in nude mice.METHODS：Peripheral blood mononuclear cells （PBMCs） were obtained from healthy volunteers by Ficoll gradient centrifugation.CIK cells were induced by culturing PBMCs in the complete medium containing recombinant human interferon-g （rhIFN-g）,anti-CD3 monoclonal antibody （mcAb）,recombinant human interleukin-2 （rhlL-2）,and recombinant human interleukin-1 （rhlL-1）.Eighty Balb/c nude mice were subcutaneously transplanted with gastric cancer cells （SGC-7901） in the exponential growth phase.Ten days later,these mice were randomly divided into four groups：control group,cisplatin group,CIK cell group and cisplatin plus CIK cell group,which were given normal saline,cisplatin,CIK cells,and cisplatin plus CIK cells for five days,respectively.RESULTS：Compared with normal saline,cisplatin and CIK cells,alone and in combination,extended the survival time of nude mice bearing tumors and decreased tumor weight （all P 〈0.01）.The reduced rate of tumor growth was significantly higher in the cisplatin plus CIK cell group （57.8%） than in the control group （P 〈0.01）.The levels of erythrocyte C3b receptor significantly increased,and those of erythrocyte immune complex receptors significantly decreased in all treatment groups when compared to the control group （all P 〈0.01）.CONCLUSION：Cisplatin and CIK cells in combination is better than alone in inhibiting the growth of human gastric cancer in nude mice.

关 键 词：细胞因子诱导的杀伤细胞 顺铂 胃癌 裸鼠 

分 类 号：R730.51[医药卫生—肿瘤]