0.2-0.4T静磁场对肿瘤细胞生长和黏附功能的影响  

Effects of exposure to static magnetic fields (0.2-0.4 T) on the growth and adhesion of tumor cells

作　　者：曹建平[1] 骞爱荣[1] 张维[1] 商澎[1] 

机构地区：[1]西北工业大学生命科学院空间生物实验模拟技术国防重点学科实验室特殊环境生物物理学研究所,陕西省西安市710072

出　　处：《世界华人消化杂志》2010年第13期1337-1343,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30670520~~

摘　　要：目的:研究0.2-0.4T中强静磁场对肿瘤细胞生长和黏附的影响.方法:用0.2-0.4T静磁场对SMMC-7721、HepG2和MCF-7细胞曝磁处理后,用噻唑蓝(MTT)法检测中强磁场对SMMC-7721、HepG2和MCF-7细胞生长增殖的影响.通过结晶紫染色检测细胞对FN黏附能力的变化,并应用流式细胞技术检测中强磁场对肿瘤细胞周期的影响.结果:中强磁场影响SMMC-7721、HepG2和MCF-7细胞的生长和黏附功能.中强磁场对不同的肿瘤细胞有不同的效应,对SMMC-7721的生长没有明显的影响,但降低SMMC-7721黏附能力(1.847±0.342vs1.094±0.33,P=0.012),与对照组相比SMMC-7721细胞周期的G2/M的百分比降低(12.05%±1.14%vs3.74%±0.87%,P=0.018).而对于MCF-7细胞,中强磁场促进细胞的增殖,增强细胞对FN的黏附能力(1.094±0.076vs2.177±0.474,P=0.017),使其G2/M的百分比降低(4.42%±1.23%vs12.04%±1.65%,P=0.004).HepG2细胞在中强磁场中,细胞的增殖受到抑制,对FN的黏附能力没有明显的变化(0.305±0.076vs0.394±0.089,P=0.467),但G2/M的百分比有所升高(1.90%±0.79%vs0.24%±0.15%,P=0.046).结论:0.2-0.4T中强静磁场对不同的肿瘤细胞有不同的影响.AIM：To investigate the effects of exposure to moderate-intensity static magnetic fields on the growth and adhesion of tumor cells.METHODS：After SMMC-7721,HepG2 and MCF-7 cells were exposed to static magnetic fields （0.2-0.4 T）,cell growth was measured by methyl thiazol tetrazolium （MTT） assay,cell ad-hesion to fibronectin （FN） was detected by crystal violet staining,and cell cycle distribution was evaluated by flow cytometry.RESULTS：The effects of exposure to static magnetic fields on different cell types differed greatly. Moderate-intensity static magnetic field exposure did not affect cell growth,but reducedcell adhesion to FN （1.847 ± 0.342 vs 1.094 ± 0.33P = 0.012） and decreased the percentage of cellsin G2/M phase （12.05 ± 1.14 vs 3.74 ± 0.87,P =0.018） in SMMC-7721 cells. In MCF-7 cells,mod-erate-intensity static magnetic field exposurepromoted cell growth,enhanced cell adhesion toFN （1.094 ± 0.076 vs 2.177 ± 0.474,P = 0.017） andincreased the percentage of cells in G2/M phase（4.42% ± 1.23% vs 12.04% ± 1.65%,P = 0.004）In HepG2 cells,cell growth was inhibited andcell cycle was blocked in G2 phase （0.305 ± 0.076vs 0.394 ± 0.089,P = 0.467） after exposure tomoderate-intensity static magnetic f ields thoughcell adhesion to FN was not significantly altered（1.90% ± 0.79% vs 0.24% ± 0.15%,P = 0.046）.CONCLUSION：Exposure to moderate-intensitystatic magnetic fields （0.2-0.4 T） exerts differenteffects on cell growth,adhesion and cell cycleprogression in different types of tumor cells.

关 键 词：中强静磁场 肿瘤细胞 增殖 黏附 细胞周期 

分 类 号：R73-36[医药卫生—肿瘤]