不同方法检测肝癌患者外周血中CD^(4+)CD25^(hi-int)Tregs水平的关系及意义  

Correlation between two different methods in detecting CD^(4+)CD25^(hi-int) Tregulatory cells in peripheral blood of patients with hepatocellular carcinoma

作　　者：王芳[1,2] 吕洪敏[2] 韩涛[2] 王鹏[2] 王凤梅[2] 朱争艳[2] 向慧玲[2] 

机构地区：[1]天津医科大学三中心临床学院,天津市300170 [2]天津市第三中心医院天津市人工细胞重点实验室,天津市300170

出　　处：《世界华人消化杂志》2010年第13期1384-1389,共6页World Chinese Journal of Digestology

基　　金：国家重点基础研究发展规划(973计划)基金资助项目;No.2007CB512801;科技部科技重大专项基金资助项目;No.2008ZX10002-005;天津市科委重点攻关专项基金资助项目;No.05YFSZSF02500;天津市科委应用基础研究计划面上基金资助项目;No.06YFJMJC13100~~

摘　　要：目的:探讨以标记CD127low和Foxp3+两种方法检测肝癌(hepatocellular carcinoma,HCC)患者外周血中CD4+CD25hi-intTregs水平的相关性,建立两者转换的回归方程.方法:流式细胞术同时用CD127low和Foxp3+两种标志物检测31例HCC患者外周血中CD4+CD25hi-intTregs,检测32例对照组CD4+CD25hi-intFoxp3+Tregs水平.结果:HCC患者与健康对照组外周血中CD4+CD25hi-intFoxp3+Tregs占CD4+T细胞的比例分别为10.33%±4.47%和7.34%±1.76%,差异有统计学意义(P<0.01).HCC患者外周血中CD4+CD25hi-intCD127lowTregs的比例为10.51%±4.78%,与Foxp3+Tregs比例呈正相关,Pearson相关系数为0.889,相关有统计学意义(P<0.01).两者曲线拟合提示幂模型是最优模型,建立回归方程Y=1.236×(x0.899)(Y为Foxp3+Tregs,x为CD127lowTregs).结论:HCC患者外周血中Tregs水平明显升高,用CD127low和Foxp3+两种方法检测HCC患者外周血Tregs水平呈正相关,两者之间数值转换可利用回归方程.AIM：To explore the correlation between CD127lowand Foxp3＋ cell labeling-based methods in detecting CD4＋CD25hi-int T regulatory cells （Tregs） in peripheral blood of hepatocellular carcinoma （HCC） and to establish a regression equation based on the percentages of CD4＋CD25hi-intTregs in total CD4＋ T cells obtained with the two methods. METHODS：The levels of CD4＋CD25hi-intTregs were detected by flow cytometry using CD-127low and Foxp3＋ markers in peripheral blood of 31 HCC patients. The levels of CD4＋CD25hi-int Foxp3＋Tregs were measured in 32 healthy con-trols. RESULTS：The percentage of CD4＋CD25hi-int Foxp3＋ Tregs in total CD4＋ T cells in peripheral blood of HCC patients was signifi cantly higher than that in healthy controls （10.33% ± 4.47% vs 7.34% ± 1.76%,P 0.01）. The percentage of CD4＋CD25hi-intCD127lowTregs in peripheral blood of HCC patients （10.51% ± 4.78%） is positively correlated with that of Foxp3＋ Tregs （r = 0.889,P 0.01）. Curve f itting showed that power model is the optimal model. According to the power model,the following regression equation was established：Y = 1.236 × （x0.899）（Y and x repre-sent the percentages of Foxp3＋Tregs and CD-127lowTregs,respectively）. CONCLUSION：Treg level is significantly elevated in peripheral blood of HCC patients. There is a positive correlation between the per-centage of CD4＋CD25hi-intFoxp3＋Tregs and that of CD4＋CD25hi-intCD127lowTregs in peripheral blood of HCC patients. The conversion between the percentage of CD4＋CD25hi-intFoxp3＋Tregs and that of CD4＋CD25hi-intCD127lowTregs can be achieved using a regression equation.

关 键 词：肝癌 CD4+CD25hi-intCD127lowTregs CD4+CD25hi-intFoxp3+Tregs 

分 类 号：R735.7[医药卫生—肿瘤]