核心蛋白聚糖协同白细胞介素24对人外周血单个核细胞作用的体外研究  

Co-effect of decorin and interleukin-24 on human PBMCs in vitro

作　　者：常江[1] 王桂琴[1] 李欣欣[2] 于培霞[1] 景刚[1] 王艳红[1] 

机构地区：[1]山西医科大学微生物学与免疫学教研室,太原030001 [2]山西医科大学第二附属医院口腔科,太原030001

出　　处：《国际生物制品学杂志》2010年第3期113-119,共7页International Journal of Biologicals

基　　金：Foundation item： Provincial Natural Science Foundation of Shanxi Province （2007011107） ; Science ＆ Technology Innovation Fund of Shanxi Medical University （01200712）

摘　　要：目的 探讨核心蛋白聚糖（decorin,DCN）联合白细胞介素24（interleukin-24,IL-24）对人外周血单个核细胞（human peripheral blood mononuclear cell,PBMC）增殖及分泌干扰素γ（Interferon-γ,IFN-γ）的影响.方法 构建重组质粒pcDNA3.1（＋）-DCN和pcDNA3.1（＋）-IL-24,并利用脂质体将两种质粒转染PBMC.根据转染质粒的不同将PBMC分为空白对照组、脂质体组、空质粒组、DCN组、IL-24组、DCN与IL-24联合组.采用四甲基偶氮唑蓝比色法检测PBMC的增殖,ELISA测定细胞培养上清中IFN-γ的表达,流式细胞技术检测PBMC表面程序性死亡分子1（PD-1）的表达.采用LSD-t检验进行统计学分析.结果 重组质粒pcDNA3.1（＋）-IL-24构建成功.与其他组相比,DCN与IL-24联合能显著提高PBMC增殖率和IFN-γ分泌量,同时也能上调PD-1的表达水平.结论 DCN与IL-24双基因联合在体外能促进PBMC的增殖,并能增强PBMC的功能.Objective To investigate the co-effect of decorin （DCN） and interleukin-24 （IL-24） on proliferation and Interferon-γ （IFN-γ） secretion of human peripheral blood mononuclear cells （PBMCs）. Methods Recombinant plasmid pcDNA3. 1 （＋）-DCN and pcDNA3. 1 （＋）-IL-24 were constructed and transfected into PBMCs by liposome. After transfected with different plasmids, the PBMCs were divided into 6 groups blank control group, Lipofectamine TM group, empty vector group, DCN group, IL-24 group, DCN and IL-24 group. PBMC proliferation was determined by methyl thiazolyl tetrazolium assay. IFN-7 expression in the supernatant was detected by ELISA. Flow cytometry was used to determine the surface expression of programmed death-1 （PD-1） on PBMCs. Statistical analysis was made using LSD-t test. Results The recombinant plasmid pcDNA3. 1 （＋）-IL-24 was constructed successfully. PBMC proliferation and IFN-7 secretion were significantly higher in DCN and IL-24 group, and the expression of PD-1 was also upregulated obviously. Conclusion The combination of DCN and IL-24 could promote PBMC proliferation and strengthen the function of PBMCs in vitro.

关 键 词：蛋白聚糖类 白细胞介素类 单核细胞 基因疗法 干扰素Ⅱ型 程序性死亡分子1 

分 类 号：R[医药卫生]