基于线粒体控制区序列分析我国马鹿亚种的系统进化关系  

Molecular Phylogeny of Subspecies of Wapiti (Cervus elaphus) in China Based on Mitochondrial Control Region Sequence

作　　者：涂剑锋[1] 杨福合[1] 邢秀梅[1] 查代明[1,2] 李一清[1] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109 [2]江苏科技大学生物与环境工程学院,江苏镇江212018

出　　处：《草食家畜》2010年第2期26-30,共5页Grass-Feeding Livestock

基　　金：国家自然科技资源平台项目(2004DKA30460)

摘　　要：测定了6个马鹿亚种和左家型马鹿线粒体控制区全序列,并结合GenBank中四川和西藏马鹿亚种序列进行分析。结果显示：马鹿亚种的控制区序列长度在916～995bp之间,碱基A＋T含量明显高于G＋C,发现117个多态位点和长为37~39 bp重复单元,平均核苷酸多样度为0.0478,这些表明我国马鹿亚种间控制区遗传多样性较为丰富。通过邻接法和最大似然法构建马鹿系统进化树,其结果表明：我国马鹿亚种明显分为两个进化枝,亚种间的进化关系与其地理位置密切相关。The complete mitochondrial control region sequences of 6 subspecies of Wapiti（Cervus elaphus） and Zuojia Wapiti were determined,and analyzed adding to Sichuan and Xizang subspecies control region sequences obtained from the GenBank.The results revealed that the length of control region ranged from 916 bp to 995 bp,and A＋T content is more higher than G＋C.The sequence alignment detected 117 polymorphic sites and repeat sequence（37～39bp）,The nucleotide diversity was 0.0478.they showed that mitochondrial genetic diversity of Wapiti was abundant in China.The phylogenetic tree based on N-J and MP methods indicatd that subspecies of Chinese Wapiti were divided into two branchs,phylogenetic relationships between subspecies correlated with its geographic setting.

关 键 词：马鹿 线粒体控制区 系统进化 

分 类 号：S865.42[农业科学—野生动物驯养]