不同钙、镁浓度对褐牙鲆幼鱼生长及SOD和CAT酶活力的影响  

Effects of Ca^(2+) and Mg^(2+) concentrations on growth,SOD and CAT enzymatic activity of juvenile Paralichthys olivaceus

作　　者：王茂林[1,2] 张秀梅[2] 黄国强[2] 张沛东[2] 李君丰[1] 

机构地区：[1]大连海洋大学农业部海洋水产增养殖学重点开放实验室,116023 [2]中国海洋大学海水养殖教育部重点实验室,青岛266003

出　　处：《渔业科学进展》2010年第3期29-36,共8页Progress in Fishery Sciences

基　　金：国家科技支撑计划课题(2006BAD09A15)资助

摘　　要：在盐度30和水温20℃条件下,配制钙、镁(1∶3)总浓度A(700mg/L)、B(1100mg/L)、C(1600mg/L,对照)、D(2200mg/L)、E(2800mg/L)的人工海水,研究了5种人工海水对褐牙鲆Paralichthys olivaceus幼鱼生长、肝脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力的影响。观察发现,褐牙鲆幼鱼初次接触高钙、镁浓度人工海水(D组和E组)时产生应激反应,鱼体呈弓形,呼吸频率降低,摄食不积极,适应7d左右恢复正常。经60d养殖实验,各处理组实验鱼食物转化效率(FCE)无显著差异(P>0.05),成活率均在90%以上。0~20d时实验鱼特定生长率(SGR)和摄食率(FI)差异显著(P<0.05),低浓度组大于高浓度组,20d后,FI无显著差异(P>0.05),40~60d时低浓度组SGR小于高浓度组。各处理组SGR和FI随钙、镁总浓度上升呈下降趋势,实验进行到60d时,低浓度组A的SGR和FI分别为高浓度组E的1.13倍和1.04倍。不同钙、镁浓度对实验鱼的免疫酶活性亦有显著影响。D组实验鱼SOD酶活力和肝比重显著高于其他各处理组;E组SOD酶活力显著低于其他实验组(P<0.05),CAT酶活力也低于其他处理组,但各组间差异不显著(P>0.05)。研究结果表明,实验初期钙、镁浓度通过影响褐牙鲆的摄食而影响其生长,低钙、镁浓度组实验鱼生长较快,高浓度组生长较慢。经60d养殖驯化,除E组外的各处理组特定生长率差异不显著,过高浓度钙、镁对褐牙鲆的免疫酶活性产生一定抑制作用。建议养殖褐牙鲆时应注意避免水体钙、镁含量过高。The effects of Ca2＋ and Mg2＋ concentrations（1∶3）in artificial seawater on growth,superoxidase dismutase（SOD）and catalase（CAT）enzymatic activity of juvenile Paralichthys olivaceus were investigated at 20℃ and salinity of 30.Five concentrations of Ca2＋ and Mg2＋ were set as follows,700（group A）,1 100（group B）,1 600（group C）,2 200（group D）,and 2 800（group E）mg/L,respectively.It was found that stress response occurred including bend of fish body and reduction of ventilation rate and food intake in juvenile fish under high Ca2＋ and Mg2＋ concentrations and the fish recovered after a week.After 60 d experiment,the survival rates of all groups were over 90%,and there were no significant differences in food conversion efficiencies（FCE）among different groups（P〉0.05）.However,the effects of Ca2＋ and Mg2＋ concentrations in artificial seawater on specific growth rates（SGR）and food intake（FI）at 020 d were significant（P〈0.05）.After 20 d,there was no significant difference in FI（P〉0.05）.During 4060 d,the SGRs in low Ca2＋ and Mg2＋ concentrations groups were lower than that in high concentrations groups.The SGR and FI of all groups decreased when Ca2＋ and Mg2＋ concentrations increased.At 60 d,the SGR and FI of group A were 1.13 and 1.04 times as that of group E,respectively.Significant influences were also observed in immuno-enzymatic activity of liver among these groups（P〈0.05）.The activity of SOD and hepatosomatic index of group D were significantly higher than those of other groups.However,the activity of SOD of group E was lower than other groups.The activity of CAT of group E was also lower than other groups but no significant difference（P〉0.05）.The results indicated that the concentrations of Ca2＋ and Mg2＋hindered the growth of juvenile P.olivaceus by changing their food intake.The fish in low Ca2＋ and Mg2＋ concentration groups grew fast and those in high Ca2＋ and Mg2＋ concentration groups grew slowly.There were no

关 键 词：褐牙鲆 钙、镁浓度 生长 SOD和CAT酶活力 

分 类 号：S965.3[农业科学—水产养殖]