RIM-19的表达对人子宫颈癌细胞移植瘤血管生成的影响  

Effect of GRIM-19 expression on angiogenesis of transplanted tumor of human cervical cancer cells

作　　者：朱靖[1] 卫莹[1] 李敏[1] 程勇[2] 徐静竹[3] 肖卫华[4] 凌斌[1] 周颖[1] 

机构地区：[1]安徽医科大学附属省立医院妇产科分子实验室.安徽省分子医学重点实验室 [2]安徽省立医院放疗科 [3]安徽医科大学 [4]中国科技大学生命科学院免疫学研究所

出　　处：《中国妇幼保健》2010年第16期2277-2279,共3页Maternal and Child Health Care of China

基　　金：安徽省自然科学基金项目(项目编号:090413117)

摘　　要：目的:通过裸鼠体内实验探讨GRIM-19对子宫颈癌细胞血管生成的影响。方法:采用western blot检测GRIM-19和VEGF的mRNA和蛋白水平表达情况。将HeLa/Control、HeLa/GRIM-19接种到裸鼠皮下检测荷瘤情况,利用免疫组化法计数微血管密度。结果:HeLa/GRIM-19细胞接种肿瘤生长速度明显减慢(P<0.01)。移植瘤蛋白检测发现GRIM-19表达升高,而VEGF的表达降低。HeLa/Control移植瘤的微血管密度是72±9.3/mm2,而HeLa/GRIM-19的微血管密度为32±4.7/mm2,两者差异有统计学意义(P<0.05)。结论:GRIM-19表达可以抑制子宫颈癌细胞的微血管生成,可能是子宫颈癌治疗的新靶点。Objective：To explore the effect of GRIM-19 expression on angiogenesis of transplanted tumor of cervical cancer cells in vivo in nude mice.Methods：The expression levels of mRNA and protein of GRIM-19 and VEGF were detected by western blot.HeLa/Control and HeLa/GRIM-19 were inoculated into skin of nude mice to detect tumor-bearing condition,the microvessel density was measured with immunohistochemical method.Results：The growth rate of HeLa/GRIM-19 transplanted tumor slowed down significantly（P0.01）.The expression of GRIM-19 protein increased,while the expression of VEGF decreased in protein detection of transplanted tumor.The microvessel density of HeLa/Control transplanted tumor was 72±9.3/mm2,while the microvessel density of HeLa/GRIM-19 was 32±4.7/mm2,there was significant difference between the two groups（P0.05）.Conclusion：The expression of GRIM-19 can inhibit the angiogenesis of microvessels of cervical cancer cells,which may be a new target for treatment of cervical cancer.

关 键 词：GRIM-19 转染 子宫颈癌 血管生成 

分 类 号：R737.33[医药卫生—肿瘤]