分子生物学技术在链霉菌分类中的应用  

Application of Molecular Biological Techniques in Streptomyces Taxonomy

作　　者：张楠[1] 张本峰[2] 于建[2] 刘训理[2] 

机构地区：[1]山东农业大学生命科学学院,泰安271018 [2]山东农业大学林学院,泰安271018

出　　处：《生物技术通报》2010年第6期85-89,99,共6页Biotechnology Bulletin

摘　　要：随着分子生物学的飞速发展,分子生物技术在链霉菌的分类方法中起到了越来越重要的作用,分子分类使链霉菌的分类从传统的表型分类进入到各种基因型分类水平,基因型分类结合表型和系统发育的特征所组成的多相分类,能更客观合理地反映生物间系统进化关系。分子分类研究主要包括(G+C)mol%测定、DNA杂交、核酸结构分析、DNA指纹图谱分析和变性梯度凝胶电泳等。With the rapid development of molecular biology,molecular biological techniques play an important roles in the taxonomy of Streptomyces.Molecular taxonomy makes the Streptomyces taxonomy into various genotypic classification standards from the traditional phenotypic classification.Polyphasic taxonomy composed by genotypic classification combining with the characteristics of phenotype genotype and systematic growth can more objectively and reasonably reflect relations of biological evolution between the creatures.The methods of molecular taxonomy mainly include（G＋C）mol%,DNA hybridization,DNA/RNA structure,DNA finger printing and denaturing gradient gel electrophoresis.

关 键 词：链霉菌 分子生物学技术 分子分类 

分 类 号：Q939[生物学—微生物学]