胃癌组织中KISS-1和MMP-9的表达及其意义  

Expression and significance of KISS-1 and MMP-9 in gastric carcinoma

作　　者：王洪兴[1] 秦川[1] 张贵虎 张洁[1] 李新强[1] 李娜[1] 

机构地区：[1]新乡医学院,河南新乡453003 [2]平顶山市电业局职工医院

出　　处：《山东医药》2010年第24期9-11,共3页Shandong Medical Journal

基　　金：河南省重点学科开放课题资助项目(ZD200957)

摘　　要：目的探讨肿瘤转移抑制基因K ISS-1及基质金属蛋白酶9(MMP-9)与胃癌侵袭、转移的关系,为研究胃癌的转移机制及治疗提供理论基础。方法采用逆转录聚合酶链反应(RT-PCR)检测36例胃癌组织及36例正常胃组织中K ISS-1 mRNA及MMP-9 mRNA的表达情况,分析其与胃癌患者各临床病理指标的关系及二者的相关性。结果 K ISS-1 mRNA在胃癌组织中的阳性表达率及表达水平均低于正常胃组织(P均<0.01),并且其低表达与淋巴结转移密切相关(P<0.05);MMP-9 mRNA在胃癌组织中的阳性表达率及表达水平均高于正常胃组织(P均<0.05),MMP-9 mRNA的高表达与癌的浸润深度和淋巴结转移密切相关(P均<0.05);K ISS-1与MMP-9表达呈负相关(P<0.05)。结论 K ISS-1表达缺失和MMP-9过表达可能与胃癌的侵袭相关。Objective To explore relationship between metastasis suppressor gene KISS-1,matrix metelloproteinases 9（MMP-9） and the invasion and metastasis of gastric carcinoma,then to provide a theoretical foundation for investigation of metastatic mechanism of gastric carcinoma.Methods Reverse transcription-polymerase chain reaction（RT-PCR） method was used to detect KISS-1 mRNA and MMP-9 mRNA expression in 36 cases of gastric carcinoma and 36 cases of normal gastric epithelia.Results The positive expression rate and relative level of KISS-1 mRNA in gastric carcinoma were significantly lower than those in normal gastric mucosa（P0.01）.The low expression of KISS-1 mRNA in gastric carcinoma correlated with lymph node metastasis（P0.05）.The positive expression rate and relative level of MMP-9 mRNA in gastric carcinoma were significantly higher than those in normal gastric mucosa（P0.05）.The high expression of MMP-9 mRNA in gastric carcinoma correlated with the depth of invasion and the metastasis of lymph node（P0.05）.The expression of KISS-1 mRNA had negative correlation with the expression of MMP-9 mRNA.Conclusion Low-expression of KISS-1 and over-expression of MMP-9 may be corrrelated with invasion of gastric carcinoma.

关 键 词：胃肿瘤 KISS-1 基质金属蛋白酶9 

分 类 号：R735.2[医药卫生—肿瘤]