Galectin-1在胃癌组织中的表达及在胃癌血管生成中的作用  

Expression of Galectin-1 in gastric carcinoma and its role in tumor angiogenesis

作　　者：李富新[1] 何小玲[1] 付强[1] 王鹏志[1] 

机构地区：[1]天津医科大学总医院天津普通外科研究所,天津300052

出　　处：《山东医药》2010年第24期15-17,共3页Shandong Medical Journal

摘　　要：目的研究半乳糖凝集素1(Gal-1)在胃癌组织中的表达,并分析其与肿瘤血管生成的关系。方法采用实时荧光定量RT-PCR技术从mRNA水平检测胃癌组织及远癌切端胃组织中Gal-1的表达情况,并用免疫组织化学SABC法检测各标本中Gal-1蛋白、血管内皮生长因子(VEGF)表达及其微血管密度(MVD)。分析Gal-1、VEGF与临床病理参数及MVD之间的关系。结果胃癌组织Gal-1 mRNA及蛋白表达、VEGF表达、MVD均分别高于远癌切端胃组织(P均<0.05)。胃癌组织Gal-1 mRNA及蛋白表达、VEGF表达和MVD与浸润深度、TNM分期及淋巴结转移相关,胃癌组织Gal-1表达与VEGF、MVD呈正相关(P<0.05)。结论 Gal-1通过促进肿瘤微血管生成参与胃癌的生长和侵袭,可作为诊断及判断胃癌进展程度及预后的指标。Objective To observe the expression of Galectin-1（Gal-1） in gastric cancer and analyze its effect on the angiogenesis of gastric cancer.Methods Real time quantitative RT-PCR technology and immunohistochemistry method were used to detect the expression of Gal-1 and vascular endothelial growth factor（VEGF） and microvessel density（MVD） in gastric cancer tissues.Results The expression of Gal-1 and VEGF and MVD in gastric cancer were significantly higher than those in tissues in the surgical margin（P0.05）.There were significant correlation between the expression of Gal-1,VEGF and MVD in gastric cancer and depth of primary tumor invasion,TNM stage and lymph node metastasis（P0.05）.The expression of Gal-1 and VEGF was positively correlated with MVD in gastric cancer tissues（P0.05）.Conclusion Gal-1 plays a role in tumor progress by promoting angiogenesis of gastric cancer,which can be used to evaluate the progress and prognosis in gastric cancer.

关 键 词：胃肿瘤 半乳糖凝集素1 血管内皮生长因子 血管生成 

分 类 号：R735.2[医药卫生—肿瘤]