PI3K抑制剂LY294002对人晶状体上皮细胞E2F-1、cyclinE蛋白表达的影响  

Effect of PI3K inhibitor LY294002 on E2F-1 and cyclinE proteins in human lens epithelial cells

作　　者：李丹[1] 宁宏[1] 李婕 杨宁[1] 王思敏[1] 

机构地区：[1]中国医科大学附属第一医院,沈阳110001

出　　处：《山东医药》2010年第24期36-38,共3页Shandong Medical Journal

基　　金：辽宁省科技厅资助项目(2008225016)

摘　　要：目的探讨PI3K抑制剂LY294002对人晶状体上皮细胞(LECs)增殖及E2F-1、细胞周期素E(cyclinE)蛋白表达的影响。方法人LECs常规培养,分为实验组和对照组,实验组加入PI3K抑制剂LY294002(25μmol/L),对照组加入同体积DMSO,两组分别培养36 h后,MTT比色法测定细胞增殖状况,Western blot法检测细胞中E2F-1及cyclinE蛋白表达。结果实验组细胞增殖受到明显抑制,与对照组比较差异有统计学意义(P<0.05),人LECs中E2F-1及cyclinE蛋白表达降低(P<0.05)。结论 PI3K抑制剂LY294002可抑制人LECs的增殖,可能与下调E2F-1及cyclinE蛋白的表达水平相关。Objective To study the effect of PI3K inhibitor LY294002 on proliferation of human lens epithelial cells（LECs） and expression of E2F-1 and cyclinE proteins.Methods The human LECs were cultured normally and divided into experimental group and control group,LECs in expenmental group were treated with PI3K inhibitor LY294002（25 μmol/L） and ones in control group were treated with DMSO culture medium.After 36 hours,cell proliferation was detected by using MTT assay and the expression of E2F-1 and cyclinE proteins was assessed by method of Western blot assay.Results Compared with control group,cell proliferation of the experimental group was inhibited significantly（P 0.05）,the expressron of E2F-1 and cydinE proteins decreased significantly（P0.05）.Conclusion Cell proliferation of LECs could be inhibited by PI3K inhibitor LY294002,which was related to the decrease of E2F-1 and cyclinE proteins.

关 键 词：PI3K抑制剂 晶体 上皮细胞 细胞增殖 E2F-1蛋白 CYCLINE蛋白 

分 类 号：R776.1[医药卫生—眼科]