圆环病毒PCR技术的建立及在规模化猪场的应用  

The Establishment of Specific PCR Detecting PCV2 and its application in large-scale pig farms

作　　者：李进 管恩平[2] 秦红 房俊迪 齐春梅 

机构地区：[1]青岛出入境检验检疫局,山东青岛266000 [2]山东出入境检验检疫局,山东青岛266001 [3]青岛宝麦德生物医药科技有限公司,山东青岛266061

出　　处：《中国动物检疫》2010年第6期35-37,共3页China Animal Health Inspection

摘　　要：目的建立猪圆环病毒2型(PCV2)的快速PCR检测方法,并对规模化猪场进行流行病学调查。方法对疑似患有典型断奶仔猪多系统衰弱综合征(PMWS)的病猪进行病理学观察,针对猪圆环病毒2型开放阅读框2(ORF2)设计特异性引物进行PCR检测,建立PCR快速检测方法。对四川地区的规模化猪场进行PCV2的流行病学调查。结果建立的PCR技术可以快速检测样品中PCV2病原,可重复性好。应用此方法对9个规模化猪场的104份样品进行检测。结论 PCV2感染在规模化猪场的存在比较普遍,本研究为进一步开展疫病的检测和进行PCV2流行病学调查奠定了基础。Objective To establish a PCR method for quick detection of PCV2 and conduct epidemiological investigation in large-scale pig farms. Method Pathological examination was performed on suspected pigs with typical signs of postweaning multisystemic weak syndrome （PMWS） and a pair of specific primers was designed according to ORF2 of PCV2 to develop a PCR detection method. Result The developed PCR method could quickly detect the PCV2 in tissues.104 samples from 9 pig farms were detected by the PCR method resulting in 53.8% potitive rate. Conclusion The PCV2 exists in large-scale pig farm. This study establishes a base for further research on this virus detection and epidemiology investagation.

关 键 词：猪圆环病毒2型 断奶仔猪多系统衰弱综合征 猪圆环病毒相关疾病 聚合酶链式反应 

分 类 号：S858.258.3[农业科学—临床兽医学]