麻疯树遗传多样性的相关序列扩增多态性(SRAP)分析  

Genetic diversity of Jatropha curcas with SRAP molecular markers

作　　者：沈俊岭[1] 倪慧群[2] 陈晓阳[1,2] 黄少伟[2] 

机构地区：[1]北京林业大学林木花卉遗传育种教育部重点实验室,北京100083 [2]华南农业大学林学院,广东广州510642

出　　处：《浙江林学院学报》2010年第3期347-353,共7页Journal of Zhejiang Forestry College

基　　金："十一五"国家科技支撑计划项目(2006BAD07A04);广东省科技攻关重点项目(2007B020703003)

摘　　要：采用相关序列扩增多态性(SRAP)分子标记对中国6省(区)及印度尼西亚共8个麻疯树Jatropha curcas种源样本进行研究,旨在阐明麻疯树种源间的遗传关系和遗传多样性。结果表明:麻疯树相关序列扩增多态性-聚合酶链式反应(SRAP-PCR)最佳反应体系为:10×PCR buffer2μL,DNA模板20ng,Taq酶16.67nkat,Mg2+2.50mmol·L-1,三磷酸脱氧核苷酸(dNTP)120.00μmol·L-1,引物0.15μmol·L-1,总体积为20μL;并从274对引物中选出具有多态性的45对引物,共检测出500条清晰的条带,其中多态性条带141条,占总条带数的28.20%。非加权成对算术平均法(UPGMA)聚类分析及主成分分析表明,各个种源间的亲缘关系均很近,其相似系数为0.791～0.940,8个种源被聚为3类,其中印度尼西亚种源单独聚为1类,来自不同地域的7个中国种源被混聚为另外2类。To investigate genetic relationships and genetic diversity of Jatropha curcas,seven populations from six provinces of China and one from Indonesia were analyzed using polymerase chain reaction （PCR） with sequence related amplified polymorphic （SRAP） DNA molecular markers. The L16（45） orthogonal experimental design was utilized to optimize the SRAP amplification system with Jaccard＇s similarity coefficient as well as cluster and principle component analyses being employed. The optimum reaction concentrations in a 20 μL reaction mixture were：2 μL 10 × PCR buffer,20 ng DNA template,16.67 nkat Taq enzyme,2.50 mmol·L-1 Mg2＋,120.00 μmol·L-1 deoxyribonucleotide triphosphate（dNTP） and 0.15 μmol·L-1 primers. For the eight populations,using the 45 most informative primer pairs,141（28.20%） of which were polymorphic,SRAP screening produced 500 bands. Jaccard＇s similarity coefficient showed a high similarity （0.791 -0.940）. Also,the cluster and principle component analyses showed three groupings：the Indonesian population,and seven Chinese populations were divided into two groups.

关 键 词：林木育种学 麻疯树 相关序列扩增多态性-聚合酶链式反应 亲缘关系 聚类分析 主成分分析 

分 类 号：S722[农业科学—林木遗传育种]