HPLC法测定溶栓颗粒中阿魏酸的含量  

Determination of ferulic acid in Rongshuan grannule by HPLC

作　　者：石嫱[1] 王焕芸[2] 

机构地区：[1]武警内蒙古总队医院药剂科,内蒙古呼和浩特010040 [2]内蒙古医学院药学院,内蒙古呼和浩特010020

出　　处：《武警医学院学报》2010年第7期533-534,537,共3页Acta Academiae Medicinae CPAPF

摘　　要：【目的】采用高效液相色谱法测定溶栓颗粒中阿魏酸的含量。【方法】采用C18色谱柱Kromasil5μ100A(250mm×4.6mm,5μm);流动相:甲醇:5%醋酸溶液(24:76);流速:1.0ml/min;柱温:25℃;检测波长:323nm。【结果】阿魏酸在3.02～18.12μg/ml范围内呈良好线形关系,r=0.9999,(n=6),平均加样回收率为100.2%,RSD=1.2%(n=6)。【结论】该法可作为溶栓颗粒的质量控制方法。[Objective] To establish a HPLC method for the determination of ferulic acid in Rongshuan grannule. [Methods] The chromatographic conditions included Kromasil 5 μ 100A C18 column（250 mm × 4.6 mm, 5 μ m） and mobile phase consisting of methyl alcohol：5% acetic acid （24：76）. The flow rate was 1.0 ml/min,column temperature was at 25℃ ; detection wavelength was 323 nm. [Results] The calibration curve was linear in the range of 3.02-18.12 μ g/ml for ferulic acid, r=0.9999, （n = 6） ; the average recovery was 100.2, and RSD was 1.2% （n = 6）. [ Conclusions ]This method is available for the quality control of Rongshuan grannule.

关 键 词：溶栓颗粒 阿魏酸 高效液相色谱法 含量测定 

分 类 号：R971[医药卫生—药品]