酸性肽对体外培养大鼠星形胶质细胞增殖的影响  

Influence of acidic neuropeptide on proliferation of rat astrocytes in vitro

作　　者：马红霞[1] 周运恒[2] 范列英[1] 安玉会[3] 

机构地区：[1]同济大学附属东方医院检验科,上海200120 [2]武警上海总队医院检验科,上海201103 [3]郑州大学基础医学院生物化学与分子生物学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2010年第3期392-394,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省杰出人才创新基金资助项目012100050

摘　　要：目的:观察酸性肽对体外培养的大鼠星形胶质细胞增殖的影响。方法:SD大鼠来源的星形胶质细胞经传代纯化后分为5组培养:无血清组、体积分数20%的胎牛血清对照组和低、中及高剂量酸性肽组(分别给予37.5、75.0及150.0mg/L酸性肽),作用24、48及72h后,收集星形胶质细胞及上清液,计算细胞存活率,并采用日立7170全自动生化分析仪测定上清液中乳酸脱氢酶(LDH)的含量。另取细胞,同上分组后培养72h,采用MTT法观察细胞增殖情况,计算细胞增殖率。结果:培养24、48和72h后,5组细胞存活率及上清液中LDH含量差异均有统计学意义(细胞存活率F=149.381、58.817和119.135,LDH含量F=1247.644、2381.877和1102.340,P均<0.001);5组不同时间点间细胞存活率及上清液中LDH含量差异均有统计学意义(细胞存活率F=62.777、50.993、10.555、38.874和21.742,LDH含量F=775.762、222.799、184.471、59.806和135.954,P均<0.001)。培养72h后,5组细胞增殖率相比,差异有统计学意义(F=137.416,P<0.001)。与相同时间点的无血清组和胎牛血清对照组相比,酸性肽各组细胞存活率和增殖率均升高,上清液中LDH含量均降低(P均<0.05)。结论:酸性肽对体外培养的大鼠星形胶质细胞的增殖具有一定的营养和保护作用。Aim：To research the influence of acidic neuropeptide on the proliferation of rat astrocytes in vitro.Methods：The purified astrocytes were allocated into 5 groups：serum-free group,20% serum group and 3 different concentration of acidic neuropeptide groups（37.5,75.0 and 150.0 mg/L）. After being cultured for 24,48,and 72 h,the cells and supernatant were collected to detect the survival rate and LDH in supernatant by trypan blue dying and Rili 7170 fully automatic biochemistry analyzer,respectively. Astrocytes were allocated as above and cultured for 72 h,and the proliferation was observed by MTT assay.Results：After being cultured for 24,48,and 72 h,the survival rate and the content of LDH among the 5 groups had significant differences（the survival rate：F=149.381,58.817,and 119.135; the content of LDH：F=1 247.644,2 381.871,and 1 102.340,P0.001）.The survival rate and the content of LDH of the serum-free group,serum group,and 3 different concentration of acidic neuropeptide groups at different time points had significant differences（the survival rate：F=62.777,50.993,10.555,38.874,and 21.742;the content of LDH：F=775.762,222.799,184.471,59.806,and 135.954,P0.001）.After being cultured for 72 h,the proliferation among the 5 groups had significant differences（F=137.416,P0.001）. Compared with the serum-free group and serum group,the survival rate and proliferation rate of the acidic neuropeptide groups increased,while the content of LDH decreased（P0.05）.Conclusion：Acidic peptide may play an important role in the nutrition and protection during the proliferation and survival of astrocytes in vitro.

关 键 词：酸性肽 大鼠 星形胶质细胞 增殖 体外培养 

分 类 号：R741.02[医药卫生—神经病学与精神病学]