外源性p16基因对wtp53型人肺腺癌细胞生长的影响  

Effect of exogenous p16 gene on the growth of wild type p53 human lung adenocarcinoma cells

作　　者：付晓颖[1,2] 张颂文[1,2] 冉瑞琼[1,2] 申宗候[1,2] 顾建新[1,2] 曹世龙[1,2] 

机构地区：[1]上海医科大学肿瘤医院放疗科 [2]上海医科大学基因中心

出　　处：《中华肿瘤杂志》1999年第2期102-104,共3页Chinese Journal of Oncology

基　　金：上海市科委发展基金

摘　　要：目的探讨ｐ１６基因对人肺腺癌的作用及ｐ１６基因治疗肺腺癌的可能性。方法运用分子克隆技术构建重组人ｐ１６基因表达载体，以电穿孔法将ｐ１６基因导入到该基因缺失的ｗｔｐ５３型的人肺腺癌Ａ５４９和Ｈ４６０细胞中，得到稳定表达ｐ１６的人肺腺癌细胞。详细研究ｐ１６基因对ｗｔｐ５３型人肺腺癌细胞周期的影响和细胞增殖的作用。结果导入ｐ１６基因能使人肺腺癌Ａ５４９和Ｈ４６０细胞生长速度较对照细胞明显减慢，细胞周期阻滞在Ｇ１期，克隆形成率下降，致瘤性减少。Objective To study the effect of p16 gene on human adenocarcinoma cell lines and the posibility of exogenous p16 gene for gene therapy of lung cancer. Methods A p16 gene recombinant expression vector was constructed by cloning p16 cDNA into the pcDNA3 vector at the sites of BamH1 and Xho I, and was transferred by electroporation into p16 gene deficient human lung adenocarcinoma cell lines (A549 and H460)which expressed wild tyre p53 gene.The p16 mRNA and protein expression of A549 p16 and H460 p16 cell was detected by Northern blots and immunocytochemistry, respectively.The cell growth properties and cell cycle pattern were aassessed by flow cytometry. Results The growth and colony forming efficiency of A549 p16 and H460 p16 cells were significantly inhibited compared with the controls. Flow cytometry showed A549 p16 and H460 p16 cells were arrested in G1 phase of cell cycle, and the percentage of cells in S phase and G2/M phase was decreased significantly. Tumorigenicity of the A549 p16 and H460 p16 cells in nude mice was greatly inhibited. Conclusion The results show that human p16 gene can suppress the malignant phenotype of wild type p53 human lung adenocarcinoma cell lines (A549 and H460). p16 gene can play a role in the gene therapy of human lung adenocarcinoma.

关 键 词：P16基因 基因治疗 肺腺癌 癌细胞生长 

分 类 号：R734.205[医药卫生—肿瘤] R730.5[医药卫生—临床医学]