胃癌和癌前病变中幽门螺杆菌感染和增殖细胞核抗原、p53、c-erbB-2的表达  

Helicobacter pylori infection and expression of PCNA,p53,c erbB 2 in carcinoma and precancerours lesions of the stomach

作　　者：卢文[1] 陈丽英[1] 龚华实[1] 

机构地区：[1]福建医科大学病理学教研室

出　　处：《中华肿瘤杂志》1999年第2期125-127,共3页Chinese Journal of Oncology

基　　金：福建省科学委员会基金

摘　　要：目的探讨幽门螺杆菌（Ｈｐ）感染在胃癌发生发展中的作用。方法对７０例胃癌、６２例癌前病变和１６例对照组，应用改良Ｗａｒｔｈｉｎｓｔａｒｙ法检测Ｈｐ，免疫酶组化ＳＰ法检测增殖细胞核抗原（ＰＣＮＡ）、ｐ５３、ｃｅｒｂＢ２等基因蛋白。结果Ｈｐ感染率在胃癌和癌前病变组均高于对照组，而前两组间差异无显著性，肠型和弥漫型胃癌间Ｈｐ感染率差异亦无显著性；胃癌和癌前病变组中，Ｈｐ感染与ＰＣＮＡ表达、ｃｅｒｂＢ２过度表达及多基因过度共表达有关；ｐ５３仅在胃癌组织中表达，Ｈｐ感染者与ｐ５３过度表达有关（Ｐ＜０．０１）。结论Ｈｐ感染患者具有更多肿瘤生物学行为，包括细胞增殖加速、抑癌基因如ｐ５３失活及癌基因如ｃｅｒｂＢ２激活，在此演变过程中Ｈｐ可能作为促进剂。Objective To study the role f Helicovacter pylori (Hp) in gastric carcinoma.Methods Hp was examined in 70 patients with gastric carcinoma, 62 patients with pre malignant lesions and 16 controls by improved Warthin Starry method and PCNA, p53, cerbB 2 protein examined immunohistochemically. Results The frequency of Hp infection in gastric carcinoma and its precusor lesions was significantly higher than that in the controls.There was no significant difference in Hp infection rate between gastric carcinoma and its precursor lesions,nor between the intestinal type and diffuse type of gastric carcinoma. Hp infection correlated well with expressions of PCNA, c erbB 2 and with co expression of PCNA,p53 and c erbB 2 in gastric carcinoma and its precusors.p53 protein was expressed only in gastric carcinoma, and its expression level was higher in the presence than in the absence of Hp infection ( P <0.01). Conclusion Hp might act as a tumor promoter in the genesis of gastric carcer.

关 键 词：胃肿瘤 癌前状态 幽门螺杆菌感染 P53基因 PCNA 

分 类 号：R735.202[医药卫生—肿瘤]