骨形成蛋白7重组腺病毒转染培养兔软骨细胞的体外研究  

The expression of bone morphogenetic protein-7 recombinant adenovirus in cultured rabbit articular chondrocytes

作　　者：刘巍[1] 朱新辉[1] 崔道然[1] 崔胜宇[1] 

机构地区：[1]南通大学第二附属医院关节外科,南通226001

出　　处：《南通大学学报（医学版）》2010年第3期161-163,F0003,共4页Journal of Nantong University(Medical sciences)

基　　金：江苏省卫生厅开放课题资助项目(KF200953);南通市社会发展科技计划资助项目(S2008042)

摘　　要：目的:观察骨形成蛋白7重组腺病毒(Ad-BMP7)转染兔软骨细胞后BMP7的表达。方法:分离培养3周龄兔膝关节软骨细胞,将Ad-BMP7转入,RT-PCR检测BMP7在mRNA水平的表达,Western Blot和免疫组化染色检测其在蛋白水平的表达。另设空白对照组。结果:实验组在mRNA水平和蛋白水平均能有效表达BMP7基因。结论:携带BMP7基因的重组腺病毒体外转染的关节软骨细胞,可以产生BMP7。Objective：To investigate the expression of BMP7 in cultured rabbit chondrocytes with BMP7 gene transfected by adenovirus vector（Ad-BMP7） in vitro.Methods：Chondrocytes were isolated and cultured from a 3-week-old rabbit knee. Ad-BMP7 was transfected into the cells at the doses of 50 and 100 MOI.BMP7 mRNA expression was observed by RT-PCR and protein expression was evaluated by Western blot and immunohistochemistry.Control groups were transfected with empty adenovirus vector.Results：BMP7 mRNA expressed in experimental groups could be observed by RT-PCR. BMP7 protein could be detected in the chondrocytes by Western blot.The strong positive signal of immunohistochemistry staining for BMP7 transfected chondrocytes could be detected in experimental groups,while in the control groups weak positive immunohistochemistry staining was exhibited.Conclusion：The results suggest that chondrocytes transfected with Ad-BMP7 could produce BMP7.

关 键 词：腺病毒 骨形成蛋白7 软骨细胞 

分 类 号：R734.2[医药卫生—肿瘤]