细胞周期对Fas/APO-1在前列腺癌细胞系PC-3M中表达的影响  

Influence of cell cycle on the expression of Fas/APO 1 in the cells of PC 3M cell line

作　　者：李宏军[1,2,3] 俞莉章[1,2,3] 梁云燕 王代树 郭应禄[1,2,3] 

机构地区：[1]北京医科大学第一医院泌尿外科 [2]北京医科大学泌尿外科研究所 [3]北京肿瘤研究所

出　　处：《中华泌尿外科杂志》1999年第4期237-239,共3页Chinese Journal of Urology

摘　　要：目的研究细胞周期对ＰＣ３Ｍ细胞表面Ｆａｓ／ＡＰＯ１蛋白表达的影响。方法采用直接免疫荧光流式细胞术对前列腺癌细胞系ＰＣ３Ｍ未同步化的细胞，及经改良的笑气阻断及ＴｄＲ双阻断方法同步化的不同细胞周期细胞表面Ｆａｓ／ＡＰＯ１表达情况进行检测。结果未同步化的ＰＣ３Ｍ细胞Ｆａｓ／ＡＰＯ１表达阳性率为３４２０％；ＰＣ３Ｍ细胞系不同细胞周期的细胞表面Ｆａｓ／ＡＰＯ１表达明显不同，以Ｓ期最高达８５０５％，Ｇ１及Ｍ期较低，分别为３３２％和４９５％。结论细胞周期对细胞表面Ｆａｓ／ＡＰＯ１的表达情况有明显影响。深入研究细胞周期对肿瘤细胞Ｆａｓ／ＡＰＯ１表达情况的影响，对于采用Ｆａｓ／ＡＰＯ１系统途径治疗恶性肿瘤有重要意义。Objective To determine the influence of cell cycle on the expression of Fas/APO 1 in PC 3M cell line. Methods With direct immunofluorescence flow cytometry,expression of Fas/APO 1 in unsynchronized and synchronized cells of PC 3M cell line derived from non androgen dependent prostatic carcinoma was detected. Results Unsynchronized cells of PC 3M cell line expressed Fas/APO 1 in 34.20 %;expression levels of Fas/APO 1 in the cells of PC 3M in different cell cycles being significantly different,mainly expressed in S phase (positive rate reached 85.05%),and limitedly expressed in G 1 and M phases (positive rate was 3.32% and 4.95% respectively). Conclusions Cell cycle would influence the expression level of Fas/APO 1 significantly,and might have some clinical relevance.

关 键 词：前列腺肿瘤 细胞周期 PC-3M 表达 

分 类 号：R737.25[医药卫生—肿瘤]