雌激素对Azaserine诱导大鼠胰腺癌癌前病变DNA含量影响的实验研究  

THE EXPERIMENTAL STUDY OF ESTROGENS EFFECT ON DNA CONTENT IN PRECANCEROUS LESION OF RATS PANCREATIC CANCER INDUCED BY AZASERINE

作　　者：徐峰[1,2] 寿楠海[1,2] 姜希宏 曹伟[1,2] 徐克森 

机构地区：[1]山东医科大学附属医院普外科 [2]山东医科大学基础医学院药理学教研室

出　　处：《山东医科大学学报》1999年第1期15-17,共3页Acta Academiae Medicinae Shandong

摘　　要：为了解雌激素对大鼠胰腺腺泡细胞癌癌前病变ＤＮＡ含量的影响，对雄性Ｗｉｓｔａｒ大鼠单次腹腔注射重氮乙酰丝氨酸（Ａｚａｓｅｒｉｎｅ）３０ｍｇ／ｋｇ，部分大鼠２个月后开始肌肉注射戊酸雌二醇０．１８ｍｇ．１００ｇ－１．２ｗ－１，４个月后实验结束，应用流式细胞分析术检测大鼠胰腺组织的ＤＮＡ含量等指标。与正常对照相比，单纯给予Ａｚａｓｅｒｉｎｅ的大鼠胰腺腺泡细胞癌癌前病变ＤＮＡ含量明显增加，异倍体率５５３％；经雌激素处理的大鼠胰腺腺胞细胞癌癌前病变ＤＮＡ含量亦明显增加，异倍体率４１０３％。To learn estrogens effect on DNA content of the animal model of precancerous lesion of rats pancreatic acinar cellular cancer,azaserine injected into male wistar rats, 30mg/kg(ip).Some rats received estradiol 17β valerate(0.18mg.100g -1 .2w -1 ,im). 4 months later,rats pancreas were autopsied and DNA content was detected with FCM method. The DNA content,rate of isoploid ,SPF and PI value increase greatly in rats given azaserine while estrogen could decrease these values obviously. Azaserine could successfully induce precancerous lesion of pancreatic acinar cancer in rats, but estrogen could to some degree,inhibit this precancerous lesion from development.

关 键 词：重氮乙酰丝氨酸 雌激素 胰腺癌 DNA 癌前病变 

分 类 号：R735.9[医药卫生—肿瘤]