过氧化氢对牛脑微血管内皮细胞损伤及药物的保护作用  

DAMAGE OF BOVINE CEREBROMICROVESSEL ENDOTHELIAL CELL BY HYDROGEN PEROXIDE AND THE PROTECTION WITH DRUGS IN CULTURE

作　　者：张岫美[1,2] 魏欣冰[1,2] 孙晓红[1,2] 张敏[1,2] 吴葆杰[1,2] 

机构地区：[1]山东医科大学药理学教研室 [2]中国人民解放军464医院

出　　处：《山东医科大学学报》1999年第1期82-84,共3页Acta Academiae Medicinae Shandong

基　　金：山东省科委科研基金

摘　　要：应用牛脑微血管内皮细胞（ＢＣＭＥＣ）培养技术，研究氧自由基过氧化氢（ＨＰ）对ＢＣＭＥＣ损伤的作用。３０％ＨＰ（３μｍｏｌ／ｍｌ）与ＢＣＭＥＣ共同孵育３０ｍｉｎ，可明显损伤ＢＣＭＥＣ；甘糖酯（０．４、０．８、１．６ｍｇ／ｍｌ）可显著保护ＨＰ引起的ＢＣＭＥＣ损伤，乳酸脱氢酶（ＬＤＨ）释放减少。The injury of bovine cerebromicrovessel endothelial cells(BCMEC) by 30% hydrogen peroxide was investigated in culture. BCMECS were damaged by hydrogen peroxide at the concentration 3μmol/ml after 30 minutes incubation and the experiment showed that catalase and propylend glycol mannate sulfate (PGMS) could protect BCMEC from damage induced by hydrogen peroxide. The results suggested that the protective effects of PGMS on BCMEC injury may probably play an important role in the potential use of cerebrovascular diseases.

关 键 词：甘糖脂 BCMEC 过氧化氢 脑血管疾病 保护作用 

分 类 号：R743.05[医药卫生—神经病学与精神病学] R977.6[医药卫生—临床医学]