响应面法优化漂白浆酶水解  

Optimization of Enzymatic Hydrolysis of Bleached Pulp by Response Surface Methodology

作　　者：吕健[1] 詹怀宇[1] 付时雨[1] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640

出　　处：《中国造纸学报》2010年第2期16-21,共6页Transactions of China Pulp and Paper

基　　金：国家973计划(2007CB210201)资助课题

摘　　要：采用响应面法中的Box-Behnken实验设计,研究了温度、酶用量和pH值对漂白针叶木浆酶解效率的影响,分别提出了用于预测可溶糖转化率和葡萄糖转化率的数学模型。通过规划求解,优化了3个因素:当温度、酶用量和pH值分别为52.8℃、10g/L、5.5以及45.9℃、10g/L、5.3时,可溶糖转化率和葡萄糖转化率分别达到最大81.5%和54.3%。通过实验数据与预测数据的对比,证明模型具有准确的预测性能。Box-Behnken design in Response Surface Methodology was employed to study the effects of temperature,cellulase dosage and pH on the efficiency of enzymatic hydrolysis of bleached softwood pulp. Two mathematic models were proposed to predict the conversion rates of soluble sugar and glucose. The three parameters were optimized by solver tool in Microsoft Excel. The optimal values of temperature,cellulase dosage and pH for maximum soluble sugar conversion were found to be 52.8℃,10 g/L and 5.5,respectively. Those for maximum glucose conversion were 45.9℃,10 g/L and 5.3,respectively. Comparing the experimental data and predicted data,the models were confirmed to be of high precision.

关 键 词：纤维素 漂白浆 酶水解 响应面法 

分 类 号：TS711[轻工技术与工程—制浆造纸工程] TP27[自动化与计算机技术—检测技术与自动化装置]