植物细胞中NuMA类似蛋白的分布及其在细胞周期中的变化  

Distribution of NuMA like Proteins and Its Changes During Cell cycle in Plant Cells

作　　者：孙英丽[1] 赵允[1] 汪健[1] 翟中和[1] 

机构地区：[1]北京大学生命科学学院

出　　处：《Acta Botanica Sinica》1999年第3期235-240,共6页Acta Botanica Sinica（植物学报：英文版）

摘　　要：免疫荧光染色结果说明植物细胞核内含有与抗动物ＮｕＭＡ多抗呈阳性交叉反应的多肽。选择性抽提并结合免疫荧光染色结果说明这种多肽位于核基质纤维蛋白网络上。免疫印迹反应显示胡萝卜（ＤａｕｃｕｓｃａｒｏｔａＬ．）悬浮培养细胞核基质蛋白与抗动物ＮｕＭＡ蛋白多抗的阳性反应条带为７４ｋＤ和７６ｋＤ。有丝分裂各期免疫荧光染色的结果表明植物细胞中的ＮｕＭＡ类似蛋白在有丝分裂过程中呈现有规律的变化。结合选择性抽提的有丝分裂各期的免疫荧光染色的结果表明核基质在此过程中也发生明显变化。应用选择性抽提并结合ＤＧＤ包埋去包埋电镜技术对植物细胞间期及有丝分裂期核基质的形态结构进行了观察。结果显示胡萝卜悬浮培养细胞间期核内存在一个非染色质性的纤维蛋白网络体系，而在正处于分裂的细胞中则未观察到。以上结果说明ＮｕＭＡ类似蛋白是核基质的组分之一并与有丝分裂密切相关。Immunofluorescence staining with anti animal NuMA antibodies revealed the existence of NuMA protein like components in carrot (Daucus carota L.) suspension cells and the NuMA like proteins exhibited regular distribution during mitosis. Furthermore, immunofluorescence staining of the nuclei after cell extraction revealed that the NuMA like proteins were components of nuclear matrix proteins and the nuclear matrix changed obviously during mitosis. Western blot with anti animal NuMA antibodies identified two positive bands of the nuclear matrix components with molecular weight of 74 kD and 76 kD. Using selective extraction and diethylene glycol distearate (DGD) embedment and embedment free electron microscopy, the well organized nuclear matrix network was observed in interphase carrot cells but not in cells during metosis. These results suggest that NuMA like proteins are components of nuclear matrix, and are closely related with mitosis.

关 键 词：核基质 NuM基质 有丝分裂 植物细胞 

分 类 号：Q946.1[生物学—植物学]