小反刍兽疫病毒H、F基因及其植物表达载体相关序列克隆  

Cloning of Peste des petits ruminants virus gene H,F and their related gene sequences in plant expression vectors

作　　者：刘霞[1] 孙娟[1] 王志亮[2] 杨国锋[3] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]中国动物卫生与流行病学中心,山东青岛266032 [3]青岛农业大学生命科学学院,山东青岛266109

出　　处：《黑龙江畜牧兽医》2010年第6期26-28,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：山东省优秀中青年科学家科研奖励基金项目(BS2009SW010);青岛农业大学高层次人才启动基金项目(630717)

摘　　要：为了构建小反刍兽疫病毒(PPRV)植物表达载体,试验采用TA克隆的方法,从PPRV质粒中分别扩增出F、H基因,在H基因终止密码子前加入KDEL序列,形成H-KDEL,从烟草SRⅠ中扩增出促进外源基因表达的MAR12序列和MAR34序列,从pHL005质粒中扩增出报告基因——绿色荧光蛋白GFP基因,以上克隆载体均带有相应酶切位点。结果表明:所克隆的基因和序列大小均与理论值相符,说明各克隆载体均构建成功。To construct plant expression vectors of Peste des petits ruminants virus （PPRV）,the genes of F and H were amplified from PPRV plasmid,H-KDEL through adding KDEL was formed into the gene H before its termination codon,two sequences MAR12 and MAR34 in Tobacco SRⅠwhich enhance the expression of exogenous genes,as well as a reporter gene,green fluorescent protein gene from pHL005 plasmid was cloned by TA cloning.All the clones were with the enzyme site.The result showed that the clones size of all the genes and sequences were the same to the theories.The cloning vectors had been successfully constructed.

关 键 词：小反刍兽疫病毒 TA克隆 表达载体构建 

分 类 号：S852.65[农业科学—基础兽医学]