蛋白激酶B1基因多态性与重性抑郁障碍事件相关电位的关联性研究  

Association of PKB1 gene polymorphisms and event-related potentials in major depressive disorder

作　　者：李莉[1] 孙宁[1] 郭晋政 李爱萍[3] 张星亮 韩晋文 李永红 张克让[1] 

机构地区：[1]山西医科大学第一医院精神卫生科,太原030001 [2]忻州市荣军精神病医院 [3]山西医科大学第三医院 [4]吕梁市荣军医院 [5]太原市杏花岭区康复医院 [6]阳泉煤业(集团)有限责任公司第二医院

出　　处：《中华行为医学与脑科学杂志》2010年第6期485-488,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金面上项目（30770770）;山西省科技攻关项目（2007031091-3）;国家人事部留学归国人员科研资助项目（2007-48）

摘　　要：目的 探讨重性抑郁障碍患者脑源性神经营养因子（BDNF）磷酸肌醇3-激酶（PI3-K）途径中蛋白激酶B1（PKB1）基因多态性与事件相关电位P300、CNV检测指标的关联性.方法 采用病例对照研究,选取重性抑郁障碍患者91例和与之性别、年龄相匹配的正常对照组65例.2组均于入组当日检测其P300及CNV数据.应用聚合酶链反应（PCR）和DNA直接测序技术,检测样本的PKB1基因多态性.分析重性抑郁障碍及正常对照组间P300、CNV均数差异.比较PKB1 SNP基因型间P300及CNV指标均数的差异.结果 ①与对照组比较,重性抑郁障碍患者的P300为P2潜伏期延长（P＜0.05）;P3a、P3b及P3波幅均明显减低（P＜0.01）;CNV则为B波幅明显减低（P＜0.01）、RT反应时间明显延长（P＜0.01）.②重性抑郁障碍患者P300指标在PKB1基因rs3001371含C等位基因的C/C及C/T合并组与TT组间有统计学差别.C/C及C/T合并组P3a、P3b及P3波幅[分别为（5.93±2.35）μV,（6.51±3.00）μV,（6.27±2.43）μV],低于TT基因型组[（分别为（7.45±2.19）μV,（8.63±3.57）μV,（8.04±2.57）μV],均差异有显著性（P＜0.05）.未发现rs3001371基因型间CNV的指标均数差异有显著性（P＞0.05）.结论 重性抑郁障碍患者PKB1基因rs3001371多态性与P300主成分波幅相关联,提示遗传因素对重性抑郁障碍记忆及认知功能可能有一定的影响.Objective To explore the relationship of protein kinase B1 （ PKB1 ） gene polymorphisms in PI3-K pathway of BDNF and event-related potentials in depression. Methods The design of ease-control research was used ,aud 91 major depressive patients and 65 normal controls who were made in age and gender matched with patients were measured auditory event-related potential P300 and contingent negative variation （CNV） in the day when two groups were collected. Polymerase chain reaction （PCR） and direct DNA sequencing technology were used to detect PKB1 gene polymorphisms. Three SNPs that named rs3001371, rs2494738, rs1130214 were selected from 3 representative BLOCK Districts of PKB1. Two independent samples t test was used to analysis P300 and CNV between two groups ,and the same way to analysis the average level of P300 and CNV and PKB1 SNP genotypes. Results ① Compared with normal controls, P300 of the patients with major depressive disorder had longer latency of P2 （P 〈 0.05） and lower amplitude of P3a（P 〈 0. 01 ）, P3b （P 〈 0.01 ） and P3 （P 〈 0.01 ） ; CNV had lower amplitude of B （P 〈 0.01 ） , and longer reaction time （P 〈 0.01 ）. ②The P300 targets of Major depressive disorder had statistical difference （P 〈 0.05 ）in PKB1 rs3001371 gene between C/C and C/T genotype combined which included C allele, and T/T genotype. The amplitude of P3a （ （ 5.93 ± 2.35 ） μV, P3 b （ 6.51 ± 3.00 ） μV, P3 （ 6.27 ±2.43 ） μN） were lower than TT Genotype （ （7.45± 2.19 ） μV, （ 8.63 ± 3.57 ） μV, （ 8.04 ± 2.57 ） μV, respectively）. The mean of CNV indicators were not found different in statistics among the rs3001371 genotypes. Conclusions PKB1 gene rs3001371 polymorphism is associated with the principal component of P300 amplitude in patients with Major depressive disorder which suggest that genetic factors may have a certain impact on cognitive function in the patients with Major depressive disorder.

关 键 词：蛋白激酶B1基因 基因多态性 重性抑郁障碍 事件相关电位 

分 类 号：R749.4[医药卫生—神经病学与精神病学]